Rabbit Anti-CAV3 Monoclonal Antibody – Cat# MBS179513

Western blot analysis of Caveolin 3 expression in Human fetal heart lysate (MBS179513).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAV3

Caveolin-3 (CAV3) Antibody – Rabbit Monoclonal

Name: CAV3 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179513
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179513

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Caveolin-3 (CAV3), Monoclonal Antibody

Full Product Name

Anti-Caveolin-3 Rabbit Monoclonal Antibody

Product Gene Name

anti-CAV3 antibody

Product Synonym Names

Caveolin-3; M-caveolin; CAV3

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

17259 MW

OMIM

AF043101 mRNA

3D Structure

ModBase 3D Structure for P56539

Immunogen

A synthesized peptide derived from human Caveolin-3

Subcellular Localization

Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein. Potential hairpin-like structure in the membrane. Membrane protein of caveolae (By similarity).

Tissue Specificity

Expressed predominantly in muscle.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CAV3 antibody

Signal Transduction; Protein Trafficking; Organelle Proteins; Tags & Cell Markers; Subcellular Markers; organelles; caveolae and clathrin; Cancer; Tumor Biomarkers

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-CAV3 antibody

Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-CAV3 antibody

WB: 1:500-1:2000
IP: 1:50

Other Notes

Small volumes of anti-CAV3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CAV3 purchase

MBS179513 is an antibody targeting CAV3. Also known as Anti-Caveolin-3 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CAV3. It may not necessarily be applicable to this product.

NCBI GI #

4502589

NCBI GeneID

859

NCBI Accession #

NP_001225.1

UniProt Primary Accession #

P56539

UniProt Secondary Accession #

Q3T1A4; A8K777

UniProt Related Accession #

P56539

GenBank Nucleotide #

NM_001234.4

NCBI Official Full Name

caveolin-3

NCBI Official Synonym Full Names

caveolin 3

NCBI Official Symbol

CAV3

NCBI Official Synonym Symbols

LQT9; VIP21; LGMD1C; VIP-21

UniProt Protein Name

Caveolin-3

UniProt Synonym Protein Names

M-caveolin

Protein Family

Caveolin

UniProt Gene Name

CAV3

NCBI Protein Information

caveolin-3

NCBI Summary for CAV3

This gene encodes a caveolin family member, which functions as a component of the caveolae plasma membranes found in most cell types. Caveolin proteins are proposed to be scaffolding proteins for organizing and concentrating certain caveolin-interacting molecules. Mutations identified in this gene lead to interference with protein oligomerization or intra-cellular routing, disrupting caveolae formation and resulting in Limb-Girdle muscular dystrophy type-1C (LGMD-1C), hyperCKemia or rippling muscle disease (RMD). Alternative splicing has been identified for this locus, with inclusion or exclusion of a differentially spliced intron. In addition, transcripts utilize multiple polyA sites and contain two potential translation initiation sites. [provided by RefSeq, Jul 2008]

UniProt Comments for CAV3

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (). Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae (PubMed:19262564).

Research Articles on CAV3

1. Case series demonstrates that exercise intolerance, myalgia and rhabdomyolysis may be caused by CAV3 mutations and broadens phenotypic spectrum of caveolinopathies. Percussion-induced rapid muscle contractions were seen in 5 of 6 patients. A previously reported heterozygous mutation in CAV3 (p.T78M) and 3 novel variants (p.V14I, p.F41S, p.F54V) were identified. >50% reduction of caveolin-3 in 5 patients vs controls.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CAV3 antibody

Reference Product	PubMed Publications
DYSF antibody	>14 publications with CAV3 and DYSF
NOS3 antibody	>11 publications with CAV3 and NOS3
DES antibody	>8 publications with CAV3 and DES
NOS1 antibody	>7 publications with CAV3 and NOS1
DMD antibody	>7 publications with CAV3 and DMD
DAG1 antibody	>6 publications with CAV3 and DAG1
TRIM72 antibody	>2 publications with CAV3 and TRIM72
PFKM antibody	>1 publications with CAV3 and PFKM
PDGFRA antibody	>1 publications with CAV3 and PDGFRA

Pathways associated with anti-CAV3 antibody

Products by Pathway	Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Integrin-mediated Cell Adhesion Pathway antibodies	Integrin-mediated Cell Adhesion Pathway Diagram
PDGFR-alpha Signaling Pathway antibodies	PDGFR-alpha Signaling Pathway Diagram
Proteoglycans In Cancer Pathway antibodies	Proteoglycans In Cancer Pathway Diagram

Diseases associated with anti-CAV3 antibody

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>75 publications with CAV3 and Heart Diseases
Pain Antibodies	>46 publications with CAV3 and Pain
Muscular Dystrophies, Limb-Girdle Antibodies	>34 publications with CAV3 and Muscular Dystrophies, Limb-Girdle
Arrhythmias, Cardiac Antibodies	>33 publications with CAV3 and Arrhythmias, Cardiac
Hypertrophy Antibodies	>31 publications with CAV3 and Hypertrophy
Cardiomegaly Antibodies	>20 publications with CAV3 and Cardiomegaly
Heart Defects, Congenital Antibodies	>18 publications with CAV3 and Heart Defects, Congenital
Hypertension Antibodies	>15 publications with CAV3 and Hypertension
Inflammation Antibodies	>12 publications with CAV3 and Inflammation
Cardiomyopathy, Hypertrophic Antibodies	>9 publications with CAV3 and Cardiomyopathy, Hypertrophic

Organs/Tissues associated with anti-CAV3 antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>286 publications with CAV3 and Muscle
Embryonic Tissue Antibodies	>22 publications with CAV3 and Embryonic Tissue
Prostate Antibodies	>5 publications with CAV3 and Prostate
Connective Tissue Antibodies	>4 publications with CAV3 and Connective Tissue