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Cathepsin L/V/K/H (CTSL) Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179700

MBS179700 is an antibody targeting CTSL. Also known as Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit. Important for the overall degradation of proteins in lysosomes. More details

$210 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Cathepsin L/V/K/H (CTSL), Monoclonal Antibody
Full Product Name
Anti-Cathepsin L/V/K/H Rabbit Monoclonal Antibody
Product Gene Name
anti-CTSL antibody
Product Synonym Names
Cathepsin L1; 3.4.22.15; Cathepsin L; Major excreted protein; MEP; Cathepsin L1 heavy chain; Cathepsin L1 light chain; CTSL; CTSL1
Clonality
Monoclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Purity/Purification
Affinity-chromatography
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
37564 MW
OMIM
X12451 mRNA
3D Structure
ModBase 3D Structure for P07711
Immunogen
A synthesized peptide derived from human Cathepsin L/V/K/H
Subcellular Localization
Lysosome.
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-CTSL antibody

Neuroscience; cell adhesion proteins; membrane proteins; Signal Transduction; Cytoskeleton/ECM; Extracellular Matrix; ECM Enzymes; Other Enzymes
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-CTSL antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes for anti-CTSL antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
FC/FACS: 1:50

Western Blot (WB) of anti-CTSL antibody

Western blot analysis of Cathepsin L/V/K/H expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate; (MBS179700).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSL monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTSL
Rabbit Anti-CTSL Monoclonal Antibody – Cat# MBS179700

Other Notes

Small volumes of anti-CTSL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CTSL. It may not necessarily be applicable to this product.

NCBI GI #
384081592
NCBI GeneID
1514
NCBI Accession #
NP_001244900.1
UniProt Primary Accession #
P07711
UniProt Secondary Accession #
Q6IAV1; Q96QJ0
UniProt Related Accession #
P07711
GenBank Nucleotide #
NM_001257971.1
NCBI Official Full Name
cathepsin L1 isoform 1 preproprotein
NCBI Official Synonym Full Names
cathepsin L
NCBI Official Symbol
CTSL
NCBI Official Synonym Symbols
MEP; CATL; CTSL1
UniProt Protein Name
Cathepsin L1
UniProt Synonym Protein Names
Cathepsin L; Major excreted protein; MEP
UniProt Gene Name
CTSL
UniProt Synonym Gene Names
CTSL1; MEP
NCBI Protein Information
cathepsin L1

NCBI Summary for CTSL

The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
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UniProt Comments for CTSL

Important for the overall degradation of proteins in lysosomes.

Research Articles on CTSL

1. these findings suggest that CTSL functions as a carcinogenic factor and may contribute to Paclitaxel resistance in human ovarian cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CTSL antibody

 Reference Product  PubMed Publications
 CST3 antibody  >20 publications with CTSL and CST3
 CSTB antibody  >19 publications with CTSL and CSTB
 CSTA antibody  >18 publications with CTSL and CSTA
 COL18A1 antibody  >5 publications with CTSL and COL18A1
 SERPINB13 antibody  >4 publications with CTSL and SERPINB13
 CUX1 antibody  >4 publications with CTSL and CUX1
 SLPI antibody  >1 publications with CTSL and SLPI
 CTSC antibody  >1 publications with CTSL and CTSC
 CST7 antibody  >1 publications with CTSL and CST7

Pathways associated with anti-CTSL antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway antibodies  Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway Diagram
 Collagen Formation Pathway antibodies  Collagen Formation Pathway Diagram
 Degradation Of Collagen Pathway antibodies  Degradation Of Collagen Pathway Diagram
 Degradation Of The Extracellular Matrix Pathway antibodies  Degradation Of The Extracellular Matrix Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram

Diseases associated with anti-CTSL antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >50 publications with CTSL and Nervous System Diseases
 Gastrointestinal Diseases Antibodies  >40 publications with CTSL and Gastrointestinal Diseases
 Brain Diseases Antibodies  >32 publications with CTSL and Brain Diseases
 Liver Diseases Antibodies  >16 publications with CTSL and Liver Diseases
 Necrosis Antibodies  >16 publications with CTSL and Necrosis
 Kidney Diseases Antibodies  >14 publications with CTSL and Kidney Diseases
 Drug Toxicity Antibodies  >7 publications with CTSL and Drug Toxicity
 Atrophy Antibodies  >6 publications with CTSL and Atrophy
 Hyperplasia Antibodies  >6 publications with CTSL and Hyperplasia
 Proteinuria Antibodies  >6 publications with CTSL and Proteinuria

Organs/Tissues associated with anti-CTSL antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >67 publications with CTSL and Liver
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