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PHF19 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3209903

This is a rabbit polyclonal antibody against PHF19. It was validated on Western Blot using a cell lysate as a positive control.Target Description: PHF19 contains 2 PHD-type zinc fingers. It acts as a transcritpional repressor. Isoform 1 and isoform 2 inhibit… More details

$490 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PHF19, Polyclonal Antibody
Full Product Name
PHF19 antibody - C-terminal region
Product Gene Name
Product Synonym Gene Name
MGC131698; MGC149712; MGC149713; MGC23929; PCL3; MTF2L1
Immunogen Sequence
Synthetic peptide located within the following region: RRCIFALAVR VSLPSSPVPA SPASSSGADQ RLPSQSLSSK QKGHTWALET
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Purity/Purification
Affinity Purified
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
PHF19 peptide (MBS3234858) is used for blocking the activity of PHF19 antibody (MBS3209903)
Molecular Weight
22kDa
OMIM
609740
3D Structure
ModBase 3D Structure for Q5T6S3
Homology
Human: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human PHF19
Applications Tested/Suitable for anti-PHF19 antibody
Western Blot (WB)

Western Blot (WB) of anti-PHF19 antibody

Lanes:
1. 30 ug control HEK-293T lysate
2. 30 ug hPCL3L-DYKDDDDK transfected HEK-293T lysate
3. 30 ug hPCL3L-MYC transfected HEK-293T lysate
4. 30 ug hPCL3L-HA transfected HEK-293T lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:3000
Gene Name:
PHF19
Submitted by:
Anonymous
Rabbit Anti-PHF19 Polyclonal Antibody – Cat# MBS3209903

Western Blot (WB) of anti-PHF19 antibody

Lanes:
1. 30 ug HBEC lysate
2. 30 ug A549 lysate
3. 30 ug Calu-6 lysate
4. 30 ug NCI358 lysate
5. 30 ug NCI841 lysate
6. 30 ug NCI1299 lysate
7. 30 ug NCI1650 lysate
8. 30 ug NCI1975 lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:3000
Gene Name:
PHF19
Submitted by:
Anonymous
Rabbit Anti-PHF19 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209903

Western Blot (WB) of anti-PHF19 antibody

WB Suggested Anti-PHF19 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate
Rabbit Anti-PHF19 Polyclonal Antibody, Western Blot (WB) – Cat# MBS3209903

Other Notes

Small volumes of anti-PHF19 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PHF19 purchase

MBS3209903 is an antibody targeting PHF19. Also known as PHF19 antibody - C-terminal region. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for PHF19. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PHD finger protein 19 isoform b
NCBI Official Synonym Full Names
PHD finger protein 19
NCBI Official Symbol
PHF19
NCBI Official Synonym Symbols
PCL3; MTF2L1; TDRD19B
UniProt Protein Name
PHD finger protein 19
UniProt Synonym Protein Names
Polycomb-like protein 3
Protein Family
UniProt Gene Name
PHF19
UniProt Synonym Gene Names
PCL3; hPCL3
UniProt Entry Name
PHF19_HUMAN
NCBI Protein Information
PHD finger protein 19

UniProt Comments for PHF19

PHF19: Acts as a transcritpional repressor. Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: nucleoplasm; ESC/E(Z) complex

Molecular Function: protein binding; zinc ion binding; methylated histone residue binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; regulation of gene expression, epigenetic
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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