Rabbit Anti-S100A6 Monoclonal Antibody – Cat# MBS179792

Western blot analysis of S100 alpha 6 expression in HeLa cell lysate (MBS179792).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for S100A6

S100 Alpha 6 (S100A6) Antibody – Rabbit Monoclonal

Name: S100A6 Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179792
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179792

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

S100 alpha 6 (S100A6), Monoclonal Antibody

Full Product Name

Anti-S100 alpha 6 Rabbit Monoclonal Antibody

Product Gene Name

anti-S100A6 antibody

Product Synonym Names

Protein S100-A6; Calcyclin; Growth factor-inducible protein 2A9; MLN 4; Prolactin receptor-associated protein; PRA; S100 calcium-binding protein A6; S100A6; CACY

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

10180 MW

OMIM

M14300 mRNA

3D Structure

ModBase 3D Structure for P06703

Immunogen

A synthesized peptide derived from human S100 alpha 6

Subcellular Localization

Nucleus envelope. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-S100A6 antibody

Cell Biology; Cell Cycle; Cell Differentiation; epigenetics and nuclear Signaling

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-S100A6 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Application Notes for anti-S100A6 antibody

WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50
FC/FACS: 1:50

Other Notes

Small volumes of anti-S100A6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for S100A6 purchase

MBS179792 is an antibody targeting S100A6. Also known as Anti-S100 alpha 6 Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for S100A6. It may not necessarily be applicable to this product.

NCBI GI #

7657532

NCBI GeneID

6277

NCBI Accession #

NP_055439.1

UniProt Primary Accession #

P06703

UniProt Secondary Accession #

Q5RHS4; D3DV39

UniProt Related Accession #

P06703

GenBank Nucleotide #

NM_014624.3

NCBI Official Full Name

protein S100-A6

NCBI Official Synonym Full Names

S100 calcium binding protein A6

NCBI Official Symbol

S100A6

NCBI Official Synonym Symbols

2A9; PRA; 5B10; CABP; CACY; S10A6

UniProt Protein Name

Protein S100-A6

UniProt Synonym Protein Names

Calcyclin; Growth factor-inducible protein 2A9; MLN 4; Prolactin receptor-associated protein; PRA; S100 calcium-binding protein A6

Protein Family

Protein

UniProt Gene Name

S100A6

UniProt Synonym Gene Names

CACY; PRA

NCBI Protein Information

protein S100-A6

NCBI Summary for S100A6

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. [provided by RefSeq, Jul 2008]

Research Articles on S100A6

1. The S100A6 interacts with FOR20 and related centrosomal proteins through a conserved N-terminal domain, suggesting a novel Ca(2+)-dependent regulation of centrosomal function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-S100A6 antibody

Reference Product	PubMed Publications
S100B antibody	>70 publications with S100A6 and S100B
CACYBP antibody	>21 publications with S100A6 and CACYBP
ANXA2 antibody	>18 publications with S100A6 and ANXA2
TP53 antibody	>4 publications with S100A6 and TP53
ANXA11 antibody	>3 publications with S100A6 and ANXA11

Pathways associated with anti-S100A6 antibody

Products by Pathway	Pathway Diagram
Gastric Cancer Network 2 Pathway antibodies	Gastric Cancer Network 2 Pathway Diagram
Prostaglandin Synthesis And Regulation Pathway antibodies	Prostaglandin Synthesis And Regulation Pathway Diagram

Diseases associated with anti-S100A6 antibody

Disease Name	Pubmed Publications
Carcinoma Antibodies	>81 publications with S100A6 and Carcinoma
Neoplasms, Experimental Antibodies	>16 publications with S100A6 and Neoplasms, Experimental
Brain Diseases Antibodies	>16 publications with S100A6 and Brain Diseases
Liver Neoplasms Antibodies	>14 publications with S100A6 and Liver Neoplasms
Inflammation Antibodies	>13 publications with S100A6 and Inflammation
Breast Neoplasms Antibodies	>13 publications with S100A6 and Breast Neoplasms
Disease Models, Animal Antibodies	>12 publications with S100A6 and Disease Models, Animal
Cell Transformation, Neoplastic Antibodies	>12 publications with S100A6 and Cell Transformation, Neoplastic
Lung Neoplasms Antibodies	>12 publications with S100A6 and Lung Neoplasms
Liver Diseases Antibodies	>12 publications with S100A6 and Liver Diseases

Organs/Tissues associated with anti-S100A6 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>52 publications with S100A6 and Brain
Skin Antibodies	>44 publications with S100A6 and Skin
Muscle Antibodies	>37 publications with S100A6 and Muscle
Blood Antibodies	>33 publications with S100A6 and Blood
Lung Antibodies	>31 publications with S100A6 and Lung
Liver Antibodies	>24 publications with S100A6 and Liver
Stomach Antibodies	>18 publications with S100A6 and Stomach
Vascular Antibodies	>17 publications with S100A6 and Vascular
Bone Antibodies	>15 publications with S100A6 and Bone
Ascites Antibodies	>15 publications with S100A6 and Ascites