Rabbit Anti-ATG9A Monoclonal Antibody – Cat# MBS179984

Western blot analysis of ATG9A expression in HepG2 cell lysate (MBS179984).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG9A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATG9A

ATG9A Antibody – Rabbit Monoclonal

Name: ATG9A Antibody; Monoclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS179984
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS179984

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles… More details

$210 / 0.03 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ATG9A, Monoclonal Antibody

Full Product Name

Anti-ATG9A Rabbit Monoclonal Antibody

Product Gene Name

anti-ATG9A antibody

Product Synonym Names

Autophagy-related protein 9A; APG9-like 1; mATG9; ATG9A; APG9L1

Clonality

Monoclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Liquid

Purity/Purification

Affinity-chromatography

Preparation and Storage

Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Molecular Weight

94447 MW

OMIM

AL833865 mRNA

3D Structure

ModBase 3D Structure for Q7Z3C6

Immunogen

A synthesized peptide derived from human ATG9A

Subcellular Localization

Cytoplasmic vesicle, autophagosome membrane; Multi-pass membrane protein. Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Under amino acid starvation or rapamycin treatment, redistributes from a juxtanuclear clustered pool to a dispersed peripheral cytosolic pool. The starvation- induced redistribution depends on ULK1, ATG13, as well as SH3GLB1.

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Product Categories/Family for anti-ATG9A antibody

Cell Biology; Proteolysis/Ubiquitin; Proteasome/Ubiquitin; UB-Like Proteins; Neuroscience; Neurology Process; Neurodegenerative Disease; Cancer; Oncoproteins/Suppressors; Tumor Suppressors; Cardiovascular; Heart; Autophagy; APG Gene Products; Signal Trans

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-ATG9A antibody

Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blot (WB)

Application Notes for anti-ATG9A antibody

WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
IP: 1:50

Other Notes

Small volumes of anti-ATG9A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATG9A purchase

MBS179984 is an antibody targeting ATG9A. Also known as Anti-ATG9A Rabbit Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATG9A. It may not necessarily be applicable to this product.

NCBI GI #

116089282

NCBI GeneID

79065

NCBI Accession #

NP_001070666.1

UniProt Primary Accession #

Q7Z3C6

UniProt Secondary Accession #

Q3ZAQ6; Q6P0N7; Q7Z317; Q7Z320; Q8NDK6; Q8WU65; Q9BVL5; Q9H6L1; Q9HAG7

UniProt Related Accession #

Q7Z3C6

GenBank Nucleotide #

NM_001077198.2

NCBI Official Full Name

autophagy-related protein 9A

NCBI Official Synonym Full Names

autophagy related 9A

NCBI Official Symbol

ATG9A

NCBI Official Synonym Symbols

mATG9; APG9L1; MGD3208

UniProt Protein Name

Autophagy-related protein 9A

UniProt Synonym Protein Names

APG9-like 1; mATG9

Protein Family

Autophagy-related protein

UniProt Gene Name

ATG9A

UniProt Synonym Gene Names

APG9L1

NCBI Protein Information

autophagy-related protein 9A

Research Articles on ATG9A

1. Contrary to its normal trafficking between plasma membrane, intracellular organelles and autophagic membranes, ATG9A concentrates in transferrin receptor-positive juxtanuclear recycling endosomes in SMPD1-deficient cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ATG9A antibody

Disease Name	Pubmed Publications
Death Antibodies	>4 publications with ATG9A and Death
Cardiomegaly Antibodies	>1 publications with ATG9A and Cardiomegaly
Disease Models, Animal Antibodies	>1 publications with ATG9A and Disease Models, Animal
Immune System Diseases Antibodies	>1 publications with ATG9A and Immune System Diseases
Ovarian Diseases Antibodies	>1 publications with ATG9A and Ovarian Diseases

Organs/Tissues associated with anti-ATG9A antibody

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>2 publications with ATG9A and Heart
Kidney Antibodies	>2 publications with ATG9A and Kidney
Brain Antibodies	>2 publications with ATG9A and Brain
Bone Antibodies	>1 publications with ATG9A and Bone
Muscle Antibodies	>1 publications with ATG9A and Muscle
Nerve Antibodies	>1 publications with ATG9A and Nerve
Intestine Antibodies	>1 publications with ATG9A and Intestine
Mouth Antibodies	>1 publications with ATG9A and Mouth
Blood Antibodies	>1 publications with ATG9A and Blood
Lung Antibodies	>1 publications with ATG9A and Lung