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NHERF1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1800570

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with… More details

$345 / 0.1 mg +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
NHERF1, Monoclonal Antibody
Full Product Name
Anti-NHERF1 Purified
Product Gene Name
Product Synonym Gene Name
SLC9A3R1
Product Synonym Names
SLC9A3 regulator 1; EBP50; NPHLOP2; SLC9A3R1; SR1
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
EBP-10
Host
Mouse
Species Reactivity
Human
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Purity/Purification
Purified by Protein-A Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
The mouse monoclonal antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Preparation and Storage
Store at 2-8 degree C. Do not freeze.
OMIM
604990
3D Structure
ModBase 3D Structure for O14745
Immunogen
Bacterially produced recombinant full-length human NHERF1.
Applications Tested/Suitable for anti-NHERF1 antibody
Immunoprecipitation, Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes for anti-NHERF1 antibody
Immunohistochemistry (paraffin sections): Recommended dilution: 5 ug/ml.
Western blotting: Recommended dilution: 2 ug/ml; 60 min on vertical incubator, positive control: RAJI human lymphoma cell lysate, non-reducing conditions, 10% separating SDS-PAGE gel.

Western Blot (WB) of anti-NHERF1 antibody

Western blotting analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 ug/ml of mouse anti-NHERF1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50 kDa.
Mouse Anti-NHERF1 Monoclonal Antibody – Cat# MBS1800570

Other Notes

Small volumes of anti-NHERF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NHERF1 purchase

MBS1800570 is an antibody targeting NHERF1. Also known as Anti-NHERF1 Purified. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NHERF1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
NCBI Official Synonym Full Names
SLC9A3 regulator 1
NCBI Official Symbol
SLC9A3R1
NCBI Official Synonym Symbols
EBP50; NHERF; NHERF1; NHERF-1; NPHLOP2
UniProt Protein Name
Na(+)/H(+) exchange regulatory cofactor NHE-RF1
UniProt Synonym Protein Names
Ezrin-radixin-moesin-binding phosphoprotein 50; EBP50; Regulatory cofactor of Na(+)/H(+) exchanger; Sodium-hydrogen exchanger regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1
UniProt Gene Name
SLC9A3R1
UniProt Synonym Gene Names
NHERF; NHERF1; NHERF-1; EBP50
UniProt Entry Name
NHRF1_HUMAN
NCBI Protein Information
Na(+)/H(+) exchange regulatory cofactor NHE-RF1

NCBI Summary for NHERF1

This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.[provided by RefSeq, Sep 2009]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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