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CD57 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801575

CD57, also known as HNK1 or Leu7, is a sulphated trisaccharide (3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc) attached to several glycoproteins, including CD56, myelin glycoprotein PO, and neural cell adhesion molecule L1, as well as on glycolipids and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD57, Monoclonal Antibody
Full Product Name
Anti-Hu CD57 PE
Product Gene Name
Product Synonym Gene Name
B3GAT1
Product Synonym Names
beta-1,3-glucuronyltransferase 1; HNK1; LEU7; 3-O-sulfoglucuronic acid beta1-3 Gal beta1-4 GlcNAc
Clonality
Monoclonal
Isotype
IgM
Clone Number
TB01
Host
Mouse
Species Reactivity
Human
Form/Format
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The mouse monoclonal antibody TB01 recognizes CD57, a carbohydrate extracellular antigen present mainly on NK cells, NK T cells, and in neural tissue.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD57 Clone #TB01 (MBS1801572)
PE Conjugated Antibody: CD57 Clone #TB01 (MBS1801575)
OMIM
AB029396 mRNA
3D Structure
ModBase 3D Structure for Q9P2W7
Immunogen
A pool of neuroblastoma cell lines
Conjugation
PE
Applications Tested/Suitable for anti-CD57 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD57 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD57 antibody

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD57 (TB01) PE antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD8 (MEM-31) Pacific Blue™ antibody (4 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD57 Monoclonal Antibody – Cat# MBS1801575

Flow Cytometry (FC/FACS) of anti-CD57 antibody

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD57 (TB01) PE antibody (10 ul reagent / 100 ul of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD57 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801575

Flow Cytometry (FC/FACS) of anti-CD57 antibody

Separation of human CD57 positive NK cells (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD57 (TB01) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD57 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801575

Flow Cytometry (FC/FACS) of anti-CD57 antibody

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD57 (TB01) PE antibody (10 ul reagent / 100 ul of peripheral whole blood).
Mouse Anti-CD57 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS1801575

Other Notes

Small volumes of anti-CD57 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD57 purchase

MBS1801575 is an antibody targeting CD57. Also known as Anti-Hu CD57 PE. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD57. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform 1
NCBI Official Synonym Full Names
beta-1,3-glucuronyltransferase 1
NCBI Official Symbol
B3GAT1
NCBI Official Synonym Symbols
NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
UniProt Protein Name
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
UniProt Synonym Protein Names
Beta-1,3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P
UniProt Gene Name
B3GAT1
UniProt Synonym Gene Names
GlcUAT-P
NCBI Protein Information
galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

NCBI Summary for CD57

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD57

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Organs/Tissues associated with anti-CD57 antibody

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