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PI / RNase Staining Buffer

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS355225

Propidium iodide (or PI) is an intercalating agent and a fluorescent molecule with a molecular mass of 668.4 Da that can be used to stain cells. When excited with 488 nm wavelength light, it fluoresces red. PI binds to both DNA and RNA, so the latter must be… More details

$240 / 50 Tests +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
PI / RNase Staining Buffer
Full Product Name
PI / RNase Staining Buffer
Form/Format
In PBS (pH7.2), 0.09% NaN3
Preparation and Storage
Store undiluted at 4 degree C and protected from prolonged exposure to light. Do not freeze. Do not dilute or boil.
Usage
This reagent is used to analyze cell cycle by flow cytometry. Use 0.5 ml/test (1×106 cells) and incubate for 30 minutes at room temperature before analysis.
Applications Tested/Suitable for PI / RNase Staining Buffer buffer
Recommended Application: Flow Cytometry (FC)

Immunofluorescent Staining of PI / RNase Staining Buffer buffer

Jurkat cells were fixed with cold 70% ethanol and wash twice with cold PBS. Cells were stained with 0.5 mL of PI/RNase Staining Buffer for 30 minutes at room temperature and analyzed by flow cytometry.
PI / RNase Staining Buffer – Cat# MBS355225

Other Notes

Small volumes of PI / RNase Staining Buffer buffer vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PI / RNase Staining Buffer purchase

MBS355225 is a buffer targeting PI / RNase Staining Buffer. Also known as PI / RNase Staining Buffer .
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