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CD162 Antibody – Mouse

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1801585

CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD162, Antibody
Full Product Name
Anti-Hu CD162 APC
Product Gene Name
Product Synonym Gene Name
SELPLG
Product Synonym Names
selectin P ligand; PSGL-1; Selectin P ligand; SELPLG; CLA
Isotype
IgG1
Clone Number
TC2
Host
Mouse
Species Reactivity
Human
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Specificity
The antibody TC2 reacts with an extracellular epitope of CD162, a 220 kDa type I integral membrane protein expressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the most peripheral blood T lymphocytes, monocytes, granulocytes; it is also expressed on a subpopulation of B lymphocytes and CD34 + bone marrow cells.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.
Matching Pairs
Unconjugated Antibody: CD162 Clone #TC2 (MBS1801584)
APC Conjugated Antibody: CD162 Clone #TC2 (MBS1801585)
OMIM
600738
3D Structure
ModBase 3D Structure for Q14242
Immunogen
Human thymocytes
Conjugation
APC
Applications Tested/Suitable for anti-CD162 antibody
Flow Cytometry (FC/FACS)
Application Notes for anti-CD162 antibody
FC/FACS: QC tested

Flow Cytometry (FC/FACS) of anti-CD162 antibody

Flow cytometry analysis (surface staining) of human peripheral blood cells with anti-human CD162 (clone TC2) APC.
Mouse Anti-CD162 Antibody – Cat# MBS1801585

Other Notes

Small volumes of anti-CD162 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD162 purchase

MBS1801585 is an antibody targeting CD162. Also known as Anti-Hu CD162 APC. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD162. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
P-selectin glycoprotein ligand 1 isoform 1
NCBI Official Synonym Full Names
selectin P ligand
NCBI Official Symbol
SELPLG
NCBI Official Synonym Symbols
CLA; CD162; PSGL1; PSGL-1
UniProt Protein Name
P-selectin glycoprotein ligand 1
UniProt Synonym Protein Names
Selectin P ligand
UniProt Gene Name
SELPLG
UniProt Synonym Gene Names
PSGL-1
UniProt Entry Name
SELPL_HUMAN
NCBI Protein Information
P-selectin glycoprotein ligand 1

NCBI Summary for CD162

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
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UniProt Comments for CD162

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: blood coagulation; cell adhesion; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD162 antibody

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