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HtrA2/OMI Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200025

HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues.… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
HtrA2/Omi, Monoclonal Antibody
Full Product Name
HtrA2/Omi antibody
Product Gene Name
Product Synonym Names
High temperature requirement protein A2; HTRA2; OMI; PRSS25; HtrA2/Omi; EC 3.4.21.108; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine proteinase OMI; Serine protease 25; Serine protease HTRA2; mitochondrial; High temperature requirement protein A2
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
1B3
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
39,914 Da
OMIM
AF020760 mRNA
3D Structure
ModBase 3D Structure for O43464
Antigen Species
Human
Immunogen
Recombinant human HtrA2/Omi (134-458aa) purified from E. coli
Clone Note
Anti-human HtrA2/Omi mAb, clone 1B3, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HtrA2/Omi protein.

Product Categories/Family for anti-HtrA2/Omi antibody

Applications Tested/Suitable for anti-HtrA2/Omi antibody
ELISA (EIA), Western Blot (WB) (Cell lysate)
Application Notes for anti-HtrA2/Omi antibody
WB: 1:500 ~ 2,000.
Recommended starting dilution is 1:1,000.

Western Blot (WB) of anti-HtrA2/Omi antibody

The extracts of 293T, MCF7, HT-29, HL-60, HeLa, mouse brain, and rat brain were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HtrA2/Omi antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-HtrA2/OMI Monoclonal Antibody – Cat# MBS200025

Product References and Citations for anti-HtrA2/Omi antibody

Miller L., et al., (1999) Trends Cell Biol. 9:323-28; Zumbrunn J., et al., (1996) FABS Lett. 398:187-92; Gui-Ying N., et al., (2003) Biochem. J. 371:39-48

Other Notes

Small volumes of anti-HtrA2/Omi antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HtrA2/Omi purchase

MBS200025 is an antibody targeting HtrA2/Omi. Also known as HtrA2/Omi antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HtrA2/Omi. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine protease HTRA2, mitochondrial isoform 1 preproprotein
NCBI Official Synonym Full Names
HtrA serine peptidase 2
NCBI Official Symbol
HTRA2
NCBI Official Synonym Symbols
OMI; MGCA8; PARK13; PRSS25
UniProt Protein Name
Serine protease HTRA2, mitochondrial
UniProt Synonym Protein Names
High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI
UniProt Gene Name
HTRA2
UniProt Synonym Gene Names
OMI; PRSS25; HtrA2
NCBI Protein Information
serine protease HTRA2, mitochondrial

NCBI Summary for HtrA2/Omi

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
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UniProt Comments for HtrA2/Omi

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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