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Adiponectin (ADIPOQ) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Popular Catalog #: MBS200027

Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member). Adiponectin contains a modular structure comprising an N-terminal collagenous domain… More details

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Product Overview

Online Datasheet in PDF
Product Name
Adiponectin (ADIPOQ), Monoclonal Antibody
Full Product Name
Adiponectin antibody
Product Gene Name
Product Synonym Names
ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin globular domain; Mouse; Adipocyte; C1q and collagen domain-containing protein; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipose most abunda; Adiponectin; 30 kDa adipocyte complement related protein; ACRP 30; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipose most abundant gene transcript 1; APM 1; GBP 28; Gelatin binding protein
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
5H7
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Specificity
Can be stored at 2-8°C for 1 week. For long term storage, alqiuot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, alqiuot and store at -20°C to -80°C . Avoid repeated freezing and thawing cycles.
Molecular Weight
26,414 Da
OMIM
D45371 mRNA
3D Structure
ModBase 3D Structure for Q15848
Immunogen
Recombinant human adiponectin (15-244aa) purified from E. coli
Clone Note
Anti-human adiponectin mAb, clone 5H7, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Acrp30 protein.

Product Categories/Family for anti-ADIPOQ antibody

Applications Tested/Suitable for anti-ADIPOQ antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ADIPOQ antibody
WB: 1:1,000 ~ 3,000.
Recommended starting dilution is 1:2,000. Please note that this antibody (5H7) has the specificity against collagen-like domain of adiponectin.

Western Blot (WB) of anti-ADIPOQ antibody

The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate
Mouse Anti-ADIPOQ Monoclonal Antibody – Cat# MBS200027

Immunofluorescence (IF) of anti-ADIPOQ antibody

ICC/IF analysis of Adiponectin in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Adiponectin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-ADIPOQ Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200027

Product References and Citations for anti-ADIPOQ antibody

Maeda K. et al., (1996) Biochem Biophys Res Commun. 221:286-9.; Berg AH. et al., (2001) Nat Med; 7:947-53; Berg AH. et al., (2002) Trends Endocrinol Metab. 13: 84-89.; Yamauchi T. et al., (2002) Nat Med. 8:1288-95

Other Notes

Small volumes of anti-ADIPOQ antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADIPOQ purchase

MBS200027 is an antibody targeting ADIPOQ. Also known as Adiponectin antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ADIPOQ. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
ADIPOQ
NCBI Official Synonym Symbols
ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
UniProt Protein Name
Adiponectin
UniProt Synonym Protein Names
30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein
Protein Family
UniProt Gene Name
ADIPOQ
UniProt Synonym Gene Names
ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1
NCBI Protein Information
adiponectin

NCBI Summary for ADIPOQ

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]
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UniProt Comments for ADIPOQ

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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