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PGP9.5 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200060

PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological… More details

$285 / 0.05 mL +1 FREE 8GB USB
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Product Overview

Online Datasheet in PDF
Product Name
PGP9.5, Monoclonal Antibody
Full Product Name
PGP9.5 antibody
Product Gene Name
Product Synonym Names
ubiquitin carboxyl-terminal esterase L1; uCHL1; PGP9.5; 1-223aa; uCH-L1; EC 3.4.19.12; ubiquitin thioesterase L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy; Neuron cytoplasmic protein 9.5; Park 5; Parkinson Disease 5; PGP95; uCH L1; Protein gene product 9.5; ubiquitin C terminal esterase L1; ubiquitin C terminal hydrolase (neuron specific); ubiquitin carboxyl terminal esterase L1; ubiquitin carboxyl terminal hydrolase isozyme L1; ubiquitin thiolesterase; ubiquitin thiolesterase L1.
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
3D9
Host
Mouse
Species Reactivity
Human, Mouse
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
24,888 Da
Antigen Species
Human
Immunogen
Recombinant human PGP9.5 (1-223aa) purified from E. coli
Clone Note
Anti-human PGP9.5 mAb, clone 3D9, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGP9.5 protein.

Product Categories/Family for anti-PGP9.5 antibody

Applications Tested/Suitable for anti-PGP9.5 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes for anti-PGP9.5 antibody
Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:100~200.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.

Western Blot (WB) of anti-PGP9.5 antibody

The Cell and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGP9.5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain lysate Lane 2.: U87-MG cell lysate
Mouse Anti-PGP9.5 Monoclonal Antibody – Cat# MBS200060

Immunohistochemistry (IHC) of anti-PGP9.5 antibody

Paraffin embedded sections of human nerve tissue were incubated with anti-human PGP9.5 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Mouse Anti-PGP9.5 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200060

Immunofluorescence (IF) of anti-PGP9.5 antibody

ICC/IF analysis of PGP9.5 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGP9.5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-PGP9.5 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200060

Product References and Citations for anti-PGP9.5 antibody

kenji H et al, (1999) Am J Pathol 155 (3):711-715; Liu Y et al, (2002) Cell 111 (2):209-18; Mikako Sakurai.et al, (2006) Cell 119:162-171

Other Notes

Small volumes of anti-PGP9.5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGP9.5 purchase

MBS200060 is an antibody targeting PGP9.5. Also known as PGP9.5 antibody. Reactive in Human, Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for PGP9.5. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
PGP9.5, partial
NCBI Official Symbol
UCHL1
NCBI Official Synonym Symbols
PGP9.5
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
UniProt Gene Name
UCHL1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1

UniProt Comments for PGP9.5

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity ().
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-PGP9.5 antibody

Organs/Tissues associated with anti-PGP9.5 antibody

 Organ/Tissue Name  Pubmed Publications
 Other Antibodies  >237 publications with PGP9.5 and Other
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