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ACOT11 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200104

ACOT11 (Acyl-CoA thioesterase11), also known as BFIT, is a protein with acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Expression of this protein in mouse has been associated with obesity and alternative… More details

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Product Overview

Online Datasheet in PDF
Product Name
ACOT11, Monoclonal Antibody
Full Product Name
ACOT11 antibody
Product Gene Name
Product Synonym Names
thioesterase; adipose associated isoform BFIT2; acyl-CoA thioesterase 11; thioesterase; adipose associated; STARD14; BFIT; KIAA0707; BFIT1; THEM1; StAR-related lipid transfer (START) domain containing 14
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
J4B2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
67,152 Da
OMIM
AF416921 mRNA
3D Structure
ModBase 3D Structure for Q8WXI4
Antigen Species
Human
Immunogen
Recombinant human ACOT11 (19-250aa) purified from E. coli
Clone Note
Anti-human ACOT11 mAb, clone J4B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACOT11 protein.

Product Categories/Family for anti-ACOT11 antibody

Applications Tested/Suitable for anti-ACOT11 antibody
ELISA (EIA), WB (Cell lysate), Immunohistochemistry (IHC)
Application Notes for anti-ACOT11 antibody
Recommended dilution range for Western blot is 1:500 ~ 1:2,000 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000 and Immunohistochemistry is 1:100.

Western Blot (WB) of anti-ACOT11 antibody

Cell lysates of LNCap, Hep3B (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human ACOT11 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-ACOT11 Monoclonal Antibody – Cat# MBS200104

Immunohistochemistry (IHC) of anti-ACOT11 antibody

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human ACOT11 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Mouse Anti-ACOT11 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200104

Product References and Citations for anti-ACOT11 antibody

S. H. Adams, et al: (2001) Biochem. J. 360, 135-142

Other Notes

Small volumes of anti-ACOT11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACOT11 purchase

MBS200104 is an antibody targeting ACOT11. Also known as ACOT11 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACOT11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
acyl-coenzyme A thioesterase 11 isoform 2
NCBI Official Synonym Full Names
acyl-CoA thioesterase 11
NCBI Official Symbol
ACOT11
NCBI Official Synonym Symbols
BFIT; THEA; THEM1; STARD14
UniProt Protein Name
Acyl-coenzyme A thioesterase 11
UniProt Synonym Protein Names
Acyl-CoA thioester hydrolase 11; Adipose-associated thioesterase; Brown fat-inducible thioesterase; BFIT
UniProt Gene Name
ACOT11
UniProt Synonym Gene Names
BFIT; KIAA0707; THEA; Acyl-CoA thioesterase 11; BFIT
NCBI Protein Information
acyl-coenzyme A thioesterase 11

NCBI Summary for ACOT11

This gene encodes a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010]
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UniProt Comments for ACOT11

Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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