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Vascular Cell Adhesion Molecule 1 (VCAM1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001285

MBS2001285 is an antibody targeting VCAM1. Also known as Monoclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1). Reactive in Rabbit. Host: Mouse. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Product Name
Vascular Cell Adhesion Molecule 1 (VCAM1), Monoclonal Antibody
Full Product Name
Monoclonal Antibody to Vascular Cell Adhesion Molecule 1 (VCAM1)
Product Gene Name
anti-VCAM1 antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- IE LSGPPVNGRP VTVSCKVPNV YPFDXLEIEL LKGETMMKNK EFLEEEDKKS LETKSLEMTF IPTMEDTGKV LVCQAKLHID EMEFEPKQRQ STQPLFVNVA PRDIAVWVSP SSIVEEGRSV NMTCSSYGLP APKILWSRQL KNGDLQPLSE NTTLALISTK LEDSGIYVCE GINLAGKSRK EVELVIQVAP KDIQLTAFPS KSVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C1
Host
Mouse
Species Reactivity
Rabbit
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
1mg/ml (lot specific)
Specificity
The antibody is a mouse monoclonal antibody raised against VCAM1. It has been selected for its ability to recognize VCAM1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Molecular Weight
81,808 Da
3D Structure
ModBase 3D Structure for Q865F2
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant VCAM1 (Ile409~Thr682) expressed in E.coli.
Cross Reactivity
Rabbit
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043791
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-VCAM1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-VCAM1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-VCAM1 antibody

Western Blot: Sample: Rabbit Serum; Primary Ab: 1000 Mouse Anti-Rabbit VCAM1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Mouse Anti-VCAM1 Monoclonal Antibody – Cat# MBS2001285

Western Blot (WB) of anti-VCAM1 antibody

Western Blot: Sample: Recombinant VCAM1, Rabbit.
Mouse Anti-VCAM1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS2001285

Other Notes

Small volumes of anti-VCAM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VCAM1. It may not necessarily be applicable to this product.

NCBI GI #
126723417
NCBI GeneID
100008901
NCBI Accession #
NP_001075621.1
UniProt Primary Accession #
Q865F2
GenBank Nucleotide #
NM_001082152.1
NCBI Official Full Name
vascular cell adhesion protein 1
NCBI Official Symbol
VCAM1
NCBI Official Synonym Symbols
VCAM-1
UniProt Protein Name
Adhesion molecule VCAM-1
UniProt Synonym Protein Names
Adhesion molecule VCAM-1
Protein Family
Vascular cell adhesion protein
UniProt Gene Name
VCAM-1
NCBI Protein Information
vascular cell adhesion protein 1

NCBI Summary for VCAM1

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
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UniProt Comments for VCAM1

VCAM1: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p32-p31

Cellular Component: Golgi apparatus; extracellular space; microvillus; cell surface; apical part of cell; endoplasmic reticulum; early endosome; plasma membrane; integral to membrane; podosome; sarcolemma; filopodium; external side of plasma membrane

Molecular Function: integrin binding; amine oxidase activity; cell adhesion molecule binding

Biological Process: response to nicotine; extracellular matrix organization and biogenesis; chronic inflammatory response; regulation of immune response; viral reproduction; heart development; cytokine and chemokine mediated signaling pathway; cell-matrix adhesion; response to lipopolysaccharide; heterophilic cell adhesion; leukocyte adhesion; response to ethanol; response to zinc ion; B cell differentiation; membrane to membrane docking; response to hypoxia; positive regulation of T cell proliferation; response to ionizing radiation; amine metabolic process; cell adhesion; leukocyte tethering or rolling; response to nutrient; acute inflammatory response; aging
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Research Articles on VCAM1

1. SphK1 regulates tumor cell proliferation, apoptosis and invasion, which ultimately contributes to tumor progression and malignancy phenotype in colon cancer. FAK pathway, ICAM-1 and VCAM-1 may play critical roles in this SphK1mediated effect.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-VCAM1 antibody

 Products by Pathway  Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 Cell Adhesion Molecules (CAMs) Pathway antibodies  Cell Adhesion Molecules (CAMs) Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Leukocyte Transendothelial Migration Pathway antibodies  Leukocyte Transendothelial Migration Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram
 Malaria Pathway antibodies  Malaria Pathway Diagram

Diseases associated with anti-VCAM1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >85 publications with VCAM1 and Inflammation
 Cardiovascular Diseases Antibodies  >54 publications with VCAM1 and Cardiovascular Diseases
 Necrosis Antibodies  >50 publications with VCAM1 and Necrosis
 Atherosclerosis Antibodies  >42 publications with VCAM1 and Atherosclerosis
 Kidney Diseases Antibodies  >13 publications with VCAM1 and Kidney Diseases
 Hypertension Antibodies  >12 publications with VCAM1 and Hypertension
 Heart Diseases Antibodies  >11 publications with VCAM1 and Heart Diseases
 Myocardial Ischemia Antibodies  >9 publications with VCAM1 and Myocardial Ischemia
 Anemia Antibodies  >8 publications with VCAM1 and Anemia
 Myocardial Infarction Antibodies  >7 publications with VCAM1 and Myocardial Infarction

Organs/Tissues associated with anti-VCAM1 antibody

 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >17 publications with VCAM1 and Heart
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