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MAT2A Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200137

Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with… More details

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Product Overview

Online Datasheet in PDF
Product Name
MAT2A, Monoclonal Antibody
Full Product Name
MAT2A antibody
Product Gene Name
Product Synonym Names
Methionine adenosyltransferase II alpha; MATA2; MATII; SAMS2; Methionine adenosyltransferase II alpha; MAT2A; Methionine adenosyltransferase II; alpha AdoMet synthetase; AdoMet synthetase 2; AMS 2; MAT II; MATA 2; Methionine adenosyltransferase; Methionine adenosyltransferase 2; SAMS 2; Methionine adenosyltransferase II; S adenosylmethionine synthetase gamma form; S adenosylmethionine synthetase isoform type 2; S-adenosylmethionine synthetase isoform type 2
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT3A2
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
32,965 Da
OMIM
X68836 mRNA
3D Structure
ModBase 3D Structure for P31153
Antigen Species
Human
Immunogen
Recombinant human MAT2A (1-395aa) purified from E. coli
Clone Note
Anti-human MAT2A mAb, clone AT3A2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MAT2A protein.

Product Categories/Family for anti-MAT2A antibody

Applications Tested/Suitable for anti-MAT2A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-MAT2A antibody
WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-MAT2A antibody

Cell lysates of HepG2 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MAT2A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : Ramos cell lysate Lane 3. : 293T cell lysate Lane 4. : Jurkat cell lysate Lane 5. : NIH-3T3 cell lysate
Mouse Anti-MAT2A Monoclonal Antibody – Cat# MBS200137

Immunohistochemistry (IHC) of anti-MAT2A antibody

Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
Mouse Anti-MAT2A Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS200137

Immunofluorescence (IF) of anti-MAT2A antibody

ICC/IF analysis of MAT2A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAT2A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-MAT2A Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200137

Product References and Citations for anti-MAT2A antibody

Ramani K, et al., (2008) Hepatology 47 (2):521-531; Chen H, et al., (2007) Gastroenterology 133 (1):207-218

Other Notes

Small volumes of anti-MAT2A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MAT2A purchase

MBS200137 is an antibody targeting MAT2A. Also known as MAT2A antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for MAT2A. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
S-adenosylmethionine synthase isoform type-2
NCBI Official Synonym Full Names
methionine adenosyltransferase 2A
NCBI Official Symbol
MAT2A
NCBI Official Synonym Symbols
MATA2; MATII; SAMS2
UniProt Protein Name
S-adenosylmethionine synthase isoform type-2
UniProt Synonym Protein Names
Methionine adenosyltransferase 2; MAT 2
UniProt Gene Name
MAT2A
UniProt Synonym Gene Names
AMS2; MATA2; MAT-II
NCBI Protein Information
S-adenosylmethionine synthase isoform type-2

NCBI Summary for MAT2A

The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]

UniProt Comments for MAT2A

Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The reaction comprises two steps that are both catalyzed by the same enzyme: formation of S-adenosylmethionine (AdoMet) and triphosphate, and subsequent hydrolysis of the triphosphate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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