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Aspartate Aminotransferase 2 (AST2) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2001380
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MBS2001380 is an antibody targeting AST2. Also known as Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Aspartate Aminotransferase 2 (AST2), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Aspartate Aminotransferase 2 (AST2)
Product Gene Name
anti-AST2 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.44mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: AST2 (MBS2001380)
Biotin Conjugated Antibody: AST2 (MBS2005839)
Matching Pairs
Unconjugated Antibody: AST2 (MBS2001380)
Immunogen: AST2 (MBS2010277)
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3D Structure
ModBase 3D Structure for P05202
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human; Rat
Immunogen
Recombinant AST2 (Ser30~Lys430) expressed in E.coli (MBS2010277)
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-AST2 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP)
Application Notes for anti-AST2 antibody
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-AST2 antibody

Figure. Western Blot: Sample: Recombinant AST2, Mouse.
Rabbit Anti-AST2 Polyclonal Antibody – Cat# MBS2001380

Immunohistochemistry (IHC) of anti-AST2 antibody

DAB staining on fromalin fixed paraffin-embedded kidney tissue)
Rabbit Anti-AST2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001380

Immunohistochemistry (IHC) of anti-AST2 antibody

HE staining on IHC-P; Samples: Mouse Liver Tissue.
Rabbit Anti-AST2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001380

Immunohistochemistry (IHC) of anti-AST2 antibody

HE staining on IHC-P; Samples: Mouse Kidney Tissue.
Rabbit Anti-AST2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001380

Immunohistochemistry (IHC) of anti-AST2 antibody

DAB staining on IHC-P; Sample: Rat Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse AST2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ).
Rabbit Anti-AST2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2001380

Other Notes

Small volumes of anti-AST2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AST2. It may not necessarily be applicable to this product.

NCBI GI #
6754036
NCBI GeneID
14719
NCBI Accession #
NP_034455.1
UniProt Primary Accession #
P05202
UniProt Secondary Accession #
O09188; Q3TIP6; Q3UD91; Q5HZH5
UniProt Related Accession #
P05202
GenBank Nucleotide #
NM_010325.2
NCBI Official Full Name
aspartate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
glutamatic-oxaloacetic transaminase 2, mitochondrial
NCBI Official Symbol
Got2
NCBI Official Synonym Symbols
Got-2; Kyat4; mAspAT; FABP-pm; AL022787
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
UniProt Synonym Protein Names
Fatty acid-binding protein; FABP-1; Glutamate oxaloacetate transaminase 2; Kynurenine aminotransferase 4; Kynurenine aminotransferase IV; Kynurenine--oxoglutarate transaminase 4; Kynurenine--oxoglutarate transaminase IV; Plasma membrane-associated fatty acid-binding protein; FABPpm; Transaminase A
Protein Family
Protein
UniProt Gene Name
Got2
UniProt Synonym Gene Names
Got-2; mAspAT; FABP-1; FABPpm
NCBI Protein Information
aspartate aminotransferase, mitochondrial

UniProt Comments for AST2

GOT2: Catalyzes the irreversible transamination of the L- tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.7; Mitochondrial; Transferase

Chromosomal Location of Human Ortholog: 8 D1|8 47.79 cM

Cellular Component: cell surface; extracellular exosome; membrane; mitochondrial inner membrane; mitochondrion; myelin sheath; perikaryon; plasma membrane; protein complex

Molecular Function: amino acid binding; carboxylic acid binding; catalytic activity; enzyme binding; kynurenine-oxoglutarate transaminase activity; L-aspartate:2-oxoglutarate aminotransferase activity; phospholipid binding; protein homodimerization activity; pyridoxal phosphate binding; RNA binding; transaminase activity; transferase activity

Biological Process: amino acid metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; dicarboxylic acid metabolic process; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; lipid transport; oxaloacetate metabolic process; response to ethanol; transport
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Research Articles on AST2

1. GOT2-mediated ATP synthesis may be a pivotal mechanism underlying the protective effect of febuxostat against neurodegeneration in EA

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AST2 antibody

 Reference Product  PubMed Publications
 Mdh2 antibody  >5 publications with AST2 and Mdh2
 Mdh1 antibody  >3 publications with AST2 and Mdh1
 Slc25a13 antibody  >1 publications with AST2 and Slc25a13

Pathways associated with anti-AST2 antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Metabolism Pathway antibodies  Amino Acid Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine Biosynthesis Pathway antibodies  Arginine Biosynthesis Pathway Diagram

Diseases associated with anti-AST2 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >4 publications with AST2 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >4 publications with AST2 and Cardiovascular Diseases
 Brain Diseases Antibodies  >4 publications with AST2 and Brain Diseases
 Mental Disorders Antibodies  >2 publications with AST2 and Mental Disorders
 Liver Diseases Antibodies  >2 publications with AST2 and Liver Diseases
 Nervous System Diseases Antibodies  >2 publications with AST2 and Nervous System Diseases
 Fatty Liver Antibodies  >2 publications with AST2 and Fatty Liver
 Hypertension Antibodies  >2 publications with AST2 and Hypertension
 Cardiomyopathies Antibodies  >1 publications with AST2 and Cardiomyopathies
 Hypertrophy Antibodies  >1 publications with AST2 and Hypertrophy

Organs/Tissues associated with anti-AST2 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >9 publications with AST2 and Blood
 Liver Antibodies  >6 publications with AST2 and Liver
 Brain Antibodies  >4 publications with AST2 and Brain
 Thyroid Antibodies  >3 publications with AST2 and Thyroid
 Muscle Antibodies  >3 publications with AST2 and Muscle
 Heart Antibodies  >3 publications with AST2 and Heart
 Bladder Antibodies  >2 publications with AST2 and Bladder
 Connective Tissue Antibodies  >1 publications with AST2 and Connective Tissue
 Spinal Cord Antibodies  >1 publications with AST2 and Spinal Cord
 Stomach Antibodies  >1 publications with AST2 and Stomach
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