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HAX1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200151

HAX1 is known to associate with hematopoietic cell-specific Lyn substrate 1 (HS1), one of the substrates of receptor-coupled tyrosine kinases activated during clonal expansion and deletion in lymphoid cells. It also interacts with the product of the… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
HAX1, Monoclonal Antibody
Full Product Name
HAX1 antibody
Product Gene Name
Product Synonym Names
HCLS1 associated protein X-1; HS1BP1; HCLSBP1; HCLS1 (and PKD2) associated protein
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT3C5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
21,801 Da
OMIM
U68566 mRNA
3D Structure
ModBase 3D Structure for O00165
Antigen Species
Human
Immunogen
Recombinant human HAX1 (1-279aa) purified from E. coli
Clone Note
Anti-human HAX1 mAb, clone AT3C5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HAX1 protein.

Product Categories/Family for anti-HAX1 antibody

Applications Tested/Suitable for anti-HAX1 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes for anti-HAX1 antibody
WB: 1:1000 ~ 2000.
Recommended starting dilution is 1:1000.

Western Blot (WB) of anti-HAX1 antibody

The HeLa cell lysate (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human HAX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HAX1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Raji cell lysate
Mouse Anti-HAX1 Monoclonal Antibody – Cat# MBS200151

Immunofluorescence (IF) of anti-HAX1 antibody

ICC/IF analysis of HAX1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HAX1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-HAX1 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200151

Product References and Citations for anti-HAX1 antibody

Klein C, et al., (2007) Nat Genet. 39 (1):86-92; Yin H, et al., (2001) Cytokine. 15 (3):122-37

Other Notes

Small volumes of anti-HAX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HAX1 purchase

MBS200151 is an antibody targeting HAX1. Also known as HAX1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for HAX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
HCLS1-associated protein X-1 isoform a
NCBI Official Synonym Full Names
HCLS1 associated protein X-1
NCBI Official Symbol
HAX1
NCBI Official Synonym Symbols
SCN3; HS1BP1; HCLSBP1
UniProt Protein Name
HCLS1-associated protein X-1
UniProt Synonym Protein Names
HS1-associating protein X-1; HAX-1; HS1-binding protein 1; HSP1BP-1
UniProt Gene Name
HAX1
UniProt Synonym Gene Names
HS1BP1; HAX-1; HSP1BP-1
NCBI Protein Information
HCLS1-associated protein X-1

NCBI Summary for HAX1

The protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for HAX1

Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed:26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed:26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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