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LDHA Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200176

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
LDHA, Monoclonal Antibody
Full Product Name
LDHA antibody
Product Gene Name
Product Synonym Names
L-lactate dehydrogenase A chain; GSD11; LDH1; LDHM; PIG19; L-lactate dehydrogenase A chain Cell proliferation-inducing gene 19 protein; LDH muscle subunit; M LDH; L lactate dehydrogenase A chain; l7R2; Lactate dehydrogenase 1; A chain; Lactate dehydrogenase A; Lactate dehydrogenase A4; Lactate dehydrogenase M; LDH muscle subunit; OTTMuSP00000017774; Proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59.
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
AT1A4
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
26,712 Da
OMIM
X02152 mRNA
3D Structure
ModBase 3D Structure for P00338
Antigen Species
Human
Immunogen
Recombinant human LDHA (1-332aa) purified from E Coli
Clone Note
Anti-human LDHA mAb, clone AT1A4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human LDHA protein.

Product Categories/Family for anti-LDHA antibody

Applications Tested/Suitable for anti-LDHA antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-LDHA antibody
WB/IF: 1:500 ~ 8,000.
Recommended starting dilution is 1:1,000

Western Blot (WB) of anti-LDHA antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human LDHA antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human LDHA (1:8000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Mouse Anti-LDHA Monoclonal Antibody – Cat# MBS200176

Immunofluorescence (IF) of anti-LDHA antibody

Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).
Mouse Anti-LDHA Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200176

Product References and Citations for anti-LDHA antibody

Le A, et al. (2010) Proc Natl Acad Sci U S A, 107 (5):2037-42.; Xie H, et al. (2009) Mol Cancer Ther, 8 (3):626-35.; Toyoda K, et al. (2010) J Bacteriol, 191 (13):4251-8.

Other Notes

Small volumes of anti-LDHA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for LDHA purchase

MBS200176 is an antibody targeting LDHA. Also known as LDHA antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for LDHA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
L-lactate dehydrogenase A chain isoform 1
NCBI Official Synonym Full Names
lactate dehydrogenase A
NCBI Official Symbol
LDHA
NCBI Official Synonym Symbols
LDHM; GSD11; PIG19; HEL-S-133P
UniProt Protein Name
L-lactate dehydrogenase A chain
UniProt Synonym Protein Names
Cell proliferation-inducing gene 19 protein; LDH muscle subunit; LDH-M; Renal carcinoma antigen NY-REN-59
UniProt Gene Name
LDHA
UniProt Synonym Gene Names
LDH-A; LDH-M
NCBI Protein Information
L-lactate dehydrogenase A chain

NCBI Summary for LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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