Apoptotic Peptidase Activating Factor 1 (APAF1) Antibody – Rabbit Polyclonal

APAF: Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 10 C2|10 45.47 cM

Cellular Component: apoptosome; cytoplasm; cytosol; extracellular exosome; nucleus; protein complex

Molecular Function: ADP binding; ATP binding; caspase activator activity; heat shock protein binding; identical protein binding; nucleotide binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c; apoptosis; brain development; cardiac muscle cell apoptotic process; cellular response to transforming growth factor beta stimulus; forebrain development; multicellular organism development; neural tube closure; neuron apoptosis; positive regulation of apoptotic signaling pathway; protein homooligomerization; regulation of apoptosis