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NANS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200219

NANS (Sialic acids synthease) is an enzyme that functions in the biosynthetic pathways of sialic acids. Expressed ubiquitously, NANS enzymatically catalyzes the H2O-dependent formation of N-acetylneuraminic acid (Neu5Ac) and… More details

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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NANS, Monoclonal Antibody
Full Product Name
NANS antibody
Product Gene Name
Product Synonym Names
Sialic acid synthase; SAS; N acetylneuraminate synthase
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT1G6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
40,308 Da
OMIM
AF257466 mRNA
3D Structure
ModBase 3D Structure for Q9NR45
Antigen Species
Human
Immunogen
Recombinant human NANS (1-359aa) purified from E. coli
Clone Note
Anti-human NANS mAb, clone AT1G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NANS protein.

Product Categories/Family for anti-NANS antibody

Applications Tested/Suitable for anti-NANS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-NANS antibody
WB: 1:1,000.

Western Blot (WB) of anti-NANS antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NANS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: NANS Transfected 293T cell lysate
Mouse Anti-NANS Monoclonal Antibody – Cat# MBS200219

Immunofluorescence (IF) of anti-NANS antibody

Mouse Anti-NANS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200219

Product References and Citations for anti-NANS antibody

Milstein O., et al. (2008) J Biol Chem. 283 (49):34414-22.; Aldape MJ., et al. (2007) J Infect Dis. 195 (12):1838-45.

Other Notes

Small volumes of anti-NANS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NANS purchase

MBS200219 is an antibody targeting NANS. Also known as NANS antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NANS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
sialic acid synthase
NCBI Official Synonym Full Names
N-acetylneuraminate synthase
NCBI Official Symbol
NANS
NCBI Official Synonym Symbols
SAS; SEMDG; SEMDCG; HEL-S-100
UniProt Protein Name
Sialic acid synthase
UniProt Synonym Protein Names
N-acetylneuraminate synthase (EC:2.5.1.56); N-acetylneuraminate-9-phosphate synthase (EC:2.5.1.57); N-acetylneuraminic acid phosphate synthase; N-acetylneuraminic acid synthase
UniProt Gene Name
NANS
UniProt Synonym Gene Names
SAS
NCBI Protein Information
sialic acid synthase

NCBI Summary for NANS

This gene encodes an enzyme that functions in the biosynthetic pathways of sialic acids. In vitro, the encoded protein uses N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN), respectively; however, it exhibits much higher activity toward the Neu5Ac phosphate product. In insect cells, expression of this gene results in Neu5Ac and KDN production. This gene is related to the E. coli sialic acid synthase gene neuB, and it can partially restore sialic acid synthase activity in an E. coli neuB-negative mutant. [provided by RefSeq, Jul 2008]
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UniProt Comments for NANS

Produces N-acetylneuraminic acid (Neu5Ac) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN). Can also use N-acetylmannosamine 6-phosphate and mannose 6-phosphate as substrates to generate phosphorylated forms of Neu5Ac and KDN, respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NANS antibody

 Reference Product  PubMed Publications
 GNE antibody  >1 publications with NANS and GNE
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