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T-Box Protein 3 (TBX3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2002247
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MBS2002247 is an antibody targeting TBX3. Also known as Polyclonal Antibody to T-Box Protein 3 (TBX3). Reactive in Human. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
T-Box Protein 3 (TBX3), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to T-Box Protein 3 (TBX3)
Product Gene Name
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.33mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: TBX3 (MBS2002247)
Biotin Conjugated Antibody: TBX3 (MBS2004092)
Matching Pairs
Unconjugated Antibody: TBX3 (MBS2002247)
Immunogen: TBX3 (MBS2010025)
OMIM
AF170708 mRNA
3D Structure
ModBase 3D Structure for O15119
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant TBX3 (Leu107~Thr220 (Accession # O15119)) expressed in E.coli (MBS2010025).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-TBX3 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-TBX3 antibody
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-TBX3 antibody

Western Blot:
Sample:
Lane 1: Human HepG2 Tissue;
Lane 2: Human Lung Tissue.
Rabbit Anti-TBX3 Polyclonal Antibody – Cat# MBS2002247

Western Blot (WB) of anti-TBX3 antibody

Western Blot:
Sample: Recombinant TBX3, Human.
Rabbit Anti-TBX3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2002247

Immunohistochemistry (IHC) of anti-TBX3 antibody

DAB staining on IHC-P.
Samples: Human Tissue
Rabbit Anti-TBX3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2002247

Knockout Validation of anti-TBX3 antibody

Knockout Validation:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: TBX3 knockout HepG2 cell lysate;
Predicted MW: 79, 77, 65kd
Observed MW: 70kd
Primary Ab: 2ug/ml Rabbit Anti-Human TBX3 Antibody
Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
Rabbit Anti-TBX3 Polyclonal Antibody, Knockout Validation – Cat# MBS2002247

Other Notes

Small volumes of anti-TBX3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TBX3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
T-box transcription factor TBX3 isoform 1
NCBI Official Synonym Full Names
T-box 3
NCBI Official Symbol
TBX3
NCBI Official Synonym Symbols
UMS; XHL; TBX3-ISO
UniProt Protein Name
T-box transcription factor TBX3
UniProt Gene Name
TBX3
UniProt Synonym Gene Names
T-box protein 3
NCBI Protein Information
T-box transcription factor TBX3

NCBI Summary for TBX3

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]
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UniProt Comments for TBX3

Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Acts as a negative regulator of PML function in cellular senescence.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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