• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

SBDS Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200236

The Shwachman-Bodian-Diamond syndrome (SBDS) is 249 amino acid ribosome maturation protein. The precise function of the SBDS protein is not known but it appears to play an important role in actin cytoskeletal function and mitotic spindle stabilization. Also,… More details

$285 / 0.05 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SBDS, Monoclonal Antibody
Full Product Name
SBDS antibody
Product Gene Name
Product Synonym Names
Ribosome maturation protein SBDS; CGI-97; SDS; SWDS
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT1E8
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-G affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
28,764 Da
OMIM
AY169963 mRNA
3D Structure
ModBase 3D Structure for Q9Y3A5
Antigen Species
Human
Immunogen
Recombinant human SBDS (1-250aa) purified from E. coli
Clone Note
Anti-human SBDS mAb, clone AT1E8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SBDS protein.

Product Categories/Family for anti-SBDS antibody

Applications Tested/Suitable for anti-SBDS antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-SBDS antibody
WB: 1:3000.

Western Blot (WB) of anti-SBDS antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SBDS antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate
Mouse Anti-SBDS Monoclonal Antibody – Cat# MBS200236

Immunofluorescence (IF) of anti-SBDS antibody

ICC/IF analysis of SBDS in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SBDS antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-SBDS Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200236

Product References and Citations for anti-SBDS antibody

Boocock G.R., et al. (2003) Nat Genet 33 (1): 97-101; Orelio C., et al. (2011) PLoS ONE 6 (6): e20727; Savchenko., et al. (2005) J Biol Chem 280 (19): 19213-20

Other Notes

Small volumes of anti-SBDS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SBDS purchase

MBS200236 is an antibody targeting SBDS. Also known as SBDS antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for SBDS. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ribosome maturation protein SBDS
NCBI Official Synonym Full Names
SBDS, ribosome maturation factor
NCBI Official Symbol
SBDS
NCBI Official Synonym Symbols
SDS; SWDS; CGI-97
UniProt Protein Name
Ribosome maturation protein SBDS
UniProt Synonym Protein Names
Shwachman-Bodian-Diamond syndrome protein
UniProt Gene Name
SBDS
NCBI Protein Information
ribosome maturation protein SBDS

NCBI Summary for SBDS

This gene encodes a highly conserved protein that plays an essential role in ribosome biogenesis. The encoded protein interacts with elongation factor-like GTPase 1 to disassociate eukaryotic initiation factor 6 from the late cytoplasmic pre-60S ribosomal subunit allowing assembly of the 80S subunit. Mutations within this gene are associated with the autosomal recessive disorder Shwachman-Bodian-Diamond syndrome. This gene has a closely linked pseudogene that is distally located. [provided by RefSeq, Jan 2017]
Show more

UniProt Comments for SBDS

Required for the assembly of mature ribosomes and ribosome biogenesis. Together with EFL1, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Required for normal levels of protein synthesis. May play a role in cellular stress resistance. May play a role in cellular response to DNA damage. May play a role in cell proliferation.
Show more

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-SBDS antibody

Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000