Mouse Anti-COTL1 Monoclonal Antibody – Cat# MBS200305

The SW480 cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: COTL1 transfected 293T cell lysate The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COTL1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: PC3 cell lysate Lane 2.: A549 cell lysate Lane 3.: U87-MG cell lysate Lane 4.: HeLa cell lysate

COTL1 Antibody – Mouse Monoclonal

Name: COTL1 Antibody; Monoclonal; Mouse; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS200305
Price: 285 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200305

COTL1 (coactosin-like 1), also known as CLP, is a 142 amino acid cytoplasmic and cytoskeletal protein that belongs to the actin-binding proteins ADF family and coactosin subfamily. COTL1 is first identified in slime mold and is found at highest levels in… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

COTL1, Monoclonal Antibody

Full Product Name

COTL1 antibody

Product Gene Name

anti-COTL1 antibody

Product Synonym Names

Coactosin-like protein; CLP; FLJ43657; MGC19733

Clonality

Monoclonal

Isotype

IgG2b,k

Clone Number

AT1D6

Host

Mouse

Species Reactivity

Human

Form/Format

Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.

Purity/Purification

By protein-A affinity chromatography

Concentration

1mg/ml (lot specific)

Preparation and Storage

Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

15,945 Da

OMIM

L54057 mRNA

3D Structure

ModBase 3D Structure for Q14019

Antigen Species

Human

Immunogen

Recombinant human COTL1 (1-142aa) purified from E. coli

Clone Note

Anti-human COTL1 mAb, clone AT1D6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human COTL1 protein.

Product Categories/Family for anti-COTL1 antibody

Cytokines

Applications Tested/Suitable for anti-COTL1 antibody

ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

Application Notes for anti-COTL1 antibody

Recommended starting dilution is 1:1000.

Product References and Citations for anti-COTL1 antibody

Provost. P., et al. (2001) Biochem J 359 (1): 255-263; Dai. H., et al. (2006) Biochim Biophys Acta 1764 (11): 1688-1700; Esser. J., et al. (2010) Biochem J 425 (1): 265-274;

Other Notes

Small volumes of anti-COTL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for COTL1 purchase

MBS200305 is an antibody targeting COTL1. Also known as COTL1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for COTL1. It may not necessarily be applicable to this product.

NCBI GI #

21624607

NCBI GeneID

23406

NCBI Accession #

NP_066972

UniProt Primary Accession #

Q14019

UniProt Secondary Accession #

Q86XM5; B2RDU3; D3DUL9

UniProt Related Accession #

Q14019

GenBank Nucleotide #

NM_021149.3

NCBI Official Full Name

coactosin-like protein

NCBI Official Synonym Full Names

coactosin like F-actin binding protein 1

NCBI Official Symbol

COTL1

NCBI Official Synonym Symbols

CLP

UniProt Protein Name

Coactosin-like protein

Protein Family

Coactosin-like protein

UniProt Gene Name

COTL1

UniProt Synonym Gene Names

CLP

NCBI Protein Information

coactosin-like protein

NCBI Summary for COTL1

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has been reported to map to chromosome 17 in the Smith-Magenis syndrome region, the best alignments for this gene are to chromosome 16. The Smith-Magenis syndrome region is the site of two related pseudogenes. [provided by RefSeq, Jul 2008]

UniProt Comments for COTL1

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization. Acts as a chaperone for ALOX5 (5LO), influencing both its stability and activity in leukotrienes synthesis.

Research Articles on COTL1

1. The roles of CLP and FLAP in in cellular leukotriene biosynthesis, were studied.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-COTL1 antibody

Reference Product	PubMed Publications
ALOX5 antibody	>11 publications with COTL1 and ALOX5
ALOX5AP antibody	>3 publications with COTL1 and ALOX5AP
FSCN1 antibody	>1 publications with COTL1 and FSCN1

Pathways associated with anti-COTL1 antibody

Products by Pathway	Pathway Diagram
Immune System Pathway antibodies	Immune System Pathway Diagram
Innate Immune System Pathway antibodies	Innate Immune System Pathway Diagram
Neutrophil Degranulation Pathway antibodies	Neutrophil Degranulation Pathway Diagram

Diseases associated with anti-COTL1 antibody

Disease Name	Pubmed Publications
Lung Diseases Antibodies	>4 publications with COTL1 and Lung Diseases
Lung Neoplasms Antibodies	>3 publications with COTL1 and Lung Neoplasms
Inflammation Antibodies	>2 publications with COTL1 and Inflammation
Infertility, Female Antibodies	>1 publications with COTL1 and Infertility, Female

Organs/Tissues associated with anti-COTL1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>9 publications with COTL1 and Blood
Lung Antibodies	>7 publications with COTL1 and Lung
Kidney Antibodies	>2 publications with COTL1 and Kidney
Vascular Antibodies	>1 publications with COTL1 and Vascular
Lymph Node Antibodies	>1 publications with COTL1 and Lymph Node
Uterus Antibodies	>1 publications with COTL1 and Uterus
Prostate Antibodies	>1 publications with COTL1 and Prostate
Liver Antibodies	>1 publications with COTL1 and Liver
Embryonic Tissue Antibodies	>1 publications with COTL1 and Embryonic Tissue
Lymph Antibodies	>1 publications with COTL1 and Lymph