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Glutamate Cysteine Ligase, Catalytic (GCLC) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2003305
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MBS2003305 is an antibody targeting GCLC. Also known as Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC). Reactive in Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Glutamate Cysteine Ligase, Catalytic (GCLC), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Glutamate Cysteine Ligase, Catalytic (GCLC)
Product Gene Name
anti-GCLC antibody
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-DFENSA YVVFVVLLTR VILSYKLDFL IPLSKVDENM KVAQKRDAVL QGMFYFRKDI CKGGNAVVDG CSKAQSSSEP AAEEYTLMSI DTIINGKEGV FPGLIPILNS YLENMEVDVD TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEINYS LIWKCNQIAD ELCECPELLG SGFRKAKYSG GKSDPS
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
0.9mg/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against GCLC. It has been selected for its ability to recognize GCLC in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: GCLC (MBS2003305)
Immunogen: GCLC (MBS2009073)
Matching Pairs
Unconjugated Antibody: GCLC (MBS2003305)
APC-CY7 Conjugated Antibody: GCLC (MBS2072775)
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Molecular Weight
72,571 Da
3D Structure
ModBase 3D Structure for P97494
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant GCLC (Asp435~Ser636) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072777
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-GCLC antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-GCLC antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-GCLC antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-GCLC Polyclonal Antibody – Cat# MBS2003305

Immunohistochemistry (IHC) of anti-GCLC antibody

DABstainingonIHC-P.Samples:MouseTissue)
Rabbit Anti-GCLC Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2003305

Other Notes

Small volumes of anti-GCLC antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GCLC. It may not necessarily be applicable to this product.

NCBI GI #
33468897
NCBI GeneID
14629
NCBI Accession #
NP_034425.1
UniProt Primary Accession #
P97494
UniProt Secondary Accession #
O09166; Q8VCS5
UniProt Related Accession #
P97494
GenBank Nucleotide #
NM_010295.2
NCBI Official Full Name
glutamate--cysteine ligase catalytic subunit
NCBI Official Synonym Full Names
glutamate-cysteine ligase, catalytic subunit
NCBI Official Symbol
Gclc
NCBI Official Synonym Symbols
Glclc; GLCL-H; Ggcs-hs; D9Wsu168e
UniProt Protein Name
Glutamate--cysteine ligase catalytic subunit
UniProt Synonym Protein Names
GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase
Protein Family
Glutamate--cysteine ligase
UniProt Gene Name
Gclc
UniProt Synonym Gene Names
Glclc
NCBI Protein Information
glutamate--cysteine ligase catalytic subunit

UniProt Comments for GCLC

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.

Protein type: EC 6.3.2.2; Ligase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 9 E1|9 43.36 cM

Cellular Component: cytosol; glutamate-cysteine ligase complex

Molecular Function: ADP binding; ATP binding; coenzyme binding; glutamate binding; glutamate-cysteine ligase activity; ligase activity; magnesium ion binding; nucleotide binding; protein heterodimerization activity

Biological Process: apoptotic mitochondrial changes; cell redox homeostasis; cysteine metabolic process; glutamate metabolic process; glutathione biosynthetic process; glutathione metabolic process; L-ascorbic acid metabolic process; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of protein ubiquitination; negative regulation of transcription, DNA-dependent; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; regulation of blood vessel size; regulation of mitochondrial depolarization; response to arsenic; response to heat; response to hormone; response to nitrosative stress; response to oxidative stress; response to xenobiotic stimulus
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Research Articles on GCLC

1. Data show that the catalytic subunit of glutamate cysteine ligase (Gclc)-derived glutathione buffers reactive oxygen species (ROS), and regulates metabolic reprogramming.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GCLC antibody

 Reference Product  PubMed Publications
 Gclm antibody  >220 publications with GCLC and Gclm
 Gss antibody  >20 publications with GCLC and Gss
 Mafg antibody  >4 publications with GCLC and Mafg
 Ggt1 antibody  >1 publications with GCLC and Ggt1

Pathways associated with anti-GCLC antibody

 Products by Pathway  Pathway Diagram
 Biological Oxidations Pathway antibodies  Biological Oxidations Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Glutathione And One Carbon Metabolism Pathway antibodies  Glutathione And One Carbon Metabolism Pathway Diagram
 Glutathione Biosynthesis, Glutamate => Glutathione Pathway antibodies  Glutathione Biosynthesis, Glutamate => Glutathione Pathway Diagram
 Glutathione Biosynthesis, Glutamate => Glutathione Pathway antibodies  Glutathione Biosynthesis, Glutamate => Glutathione Pathway Diagram
 Glutathione Conjugation Pathway antibodies  Glutathione Conjugation Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram
 Glutathione Metabolism Pathway antibodies  Glutathione Metabolism Pathway Diagram

Diseases associated with anti-GCLC antibody

 Disease Name  Pubmed Publications
 Liver Diseases Antibodies  >66 publications with GCLC and Liver Diseases
 Inflammation Antibodies  >45 publications with GCLC and Inflammation
 Necrosis Antibodies  >42 publications with GCLC and Necrosis
 Cardiovascular Diseases Antibodies  >34 publications with GCLC and Cardiovascular Diseases
 Drug-Induced Liver Injury Antibodies  >31 publications with GCLC and Drug-Induced Liver Injury
 Brain Diseases Antibodies  >28 publications with GCLC and Brain Diseases
 Nervous System Diseases Antibodies  >28 publications with GCLC and Nervous System Diseases
 Poisoning Antibodies  >27 publications with GCLC and Poisoning
 Drug-Related Side Effects and Adverse Reactions Antibodies  >20 publications with GCLC and Drug-Related Side Effects and Adverse Reactions
 Fibrosis Antibodies  >17 publications with GCLC and Fibrosis

Organs/Tissues associated with anti-GCLC antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >136 publications with GCLC and Liver
 Blood Antibodies  >99 publications with GCLC and Blood
 Lung Antibodies  >62 publications with GCLC and Lung
 Brain Antibodies  >61 publications with GCLC and Brain
 Kidney Antibodies  >34 publications with GCLC and Kidney
 Heart Antibodies  >26 publications with GCLC and Heart
 Muscle Antibodies  >25 publications with GCLC and Muscle
 Skin Antibodies  >19 publications with GCLC and Skin
 Eye Antibodies  >13 publications with GCLC and Eye
 Intestine Antibodies  >11 publications with GCLC and Intestine
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