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Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2003384
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MBS2003384 is an antibody targeting UCHL1. Also known as Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Reactive in Human, Rat. Host: Rabbit. More details

$220 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
Product Gene Name
anti-UCHL1 antibody
Immunogen Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG S-QLKPMEIN P EMLNKVLAKL GVAGQWRFAD VLGLEEETLG SVPSPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLEFED GSVLKQFLSE TEKLSPEDRA KCFEKNEAIQ AAHDSVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DSLLQDAAKV CREFTEREQG EVRFSAVALC KAA
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
0.51mg/ml (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against UCHL1. It has been selected for its ability to recognize UCHL1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: UCHL1 (MBS2003384)
Biotin Conjugated Antibody: UCHL1 (MBS2002467)
Matching Pairs
Unconjugated Antibody: UCHL1 (MBS2003384)
Immunogen: UCHL1 (MBS2010035)
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Molecular Weight
24,838 Da
3D Structure
ModBase 3D Structure for Q00981
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant UCHL1 (Gln2~Ala223) expressed in E.coli.
Cross Reactivity
Human, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2078885

Product Categories/Family for anti-UCHL1 antibody

Knock-Out Validation Antibody
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-UCHL1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-UCHL1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-UCHL1 antibody

Western Blot: Sample: Human A549 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat UCHL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-UCHL1 Polyclonal Antibody – Cat# MBS2003384

Western Blot (WB) of anti-UCHL1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-UCHL1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2003384

Immunohistochemistry (IHC) of anti-UCHL1 antibody

DAB staining on IHC-P. Samples: Rat Tissue)
Rabbit Anti-UCHL1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2003384

Knockout Validation of anti-UCHL1 antibody

Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: UCHL1 knockout A549 cell lysate; Predicted MW: 25kd Observed MW: 26kd Primary Ab: 1ug/ml Rabbit Anti-Rat UCHL1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
Rabbit Anti-UCHL1 Polyclonal Antibody, Knockout Validation – Cat# MBS2003384

Other Notes

Small volumes of anti-UCHL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UCHL1. It may not necessarily be applicable to this product.

NCBI GI #
61098212
NCBI GeneID
29545
NCBI Accession #
NP_058933.2
UniProt Primary Accession #
Q00981
UniProt Secondary Accession #
Q6P9V8
UniProt Related Accession #
Q00981
GenBank Nucleotide #
NM_017237.3
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase isozyme L1
NCBI Official Synonym Full Names
ubiquitin C-terminal hydrolase L1
NCBI Official Symbol
Uchl1
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase isozyme L1
UniProt Synonym Protein Names
Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1
Protein Family
Ubiquitin carboxyl-terminal hydrolase
UniProt Gene Name
Uchl1
UniProt Synonym Gene Names
UCH-L1; PGP9.5
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase isozyme L1

NCBI Summary for UCHL1

catalyzes the hydrolysis of ubiquitin carboxy-terminal thiolesters to form ubiquitin and a thiol; may play a role in neuropathic pain [RGD, Feb 2006]

UniProt Comments for UCHL1

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 14p11

Cellular Component: axon; cell soma; cytoplasm; cytosol; endoplasmic reticulum membrane; myelin sheath; nucleoplasm; plasma membrane

Molecular Function: alpha-2A adrenergic receptor binding; cysteine-type endopeptidase activity; ligase activity; omega peptidase activity; thiol-dependent ubiquitin-specific protease activity; ubiquitin binding; ubiquitin protein ligase binding

Biological Process: adult walking behavior; axon target recognition; axonal transport of mitochondrion; axonogenesis; cell proliferation; eating behavior; muscle fiber development; negative regulation of MAP kinase activity; neuromuscular process; protein deubiquitination; sensory perception of pain; ubiquitin-dependent protein catabolic process
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Research Articles on UCHL1

1. investigated temporal profile of astrocytic and neuronal injury markers after TBI; different mechanisms underlie clearance of UCH-L1 and GFAP in CSF and serum

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-UCHL1 antibody

 Reference Product  PubMed Publications
 Snca antibody  >26 publications with UCHL1 and Snca
 Lamp2 antibody  >1 publications with UCHL1 and Lamp2

Pathways associated with anti-UCHL1 antibody

 Products by Pathway  Pathway Diagram
 Parkinson's Disease Pathway antibodies  Parkinson's Disease Pathway Diagram
 Proteasome Degradation Pathway antibodies  Proteasome Degradation Pathway Diagram

Diseases associated with anti-UCHL1 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >215 publications with UCHL1 and Nervous System Diseases
 Brain Diseases Antibodies  >159 publications with UCHL1 and Brain Diseases
 Movement Disorders Antibodies  >99 publications with UCHL1 and Movement Disorders
 Inflammation Antibodies  >47 publications with UCHL1 and Inflammation
 Disease Models, Animal Antibodies  >35 publications with UCHL1 and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >32 publications with UCHL1 and Cardiovascular Diseases
 Peripheral Nervous System Diseases Antibodies  >31 publications with UCHL1 and Peripheral Nervous System Diseases
 Necrosis Antibodies  >23 publications with UCHL1 and Necrosis
 Nerve Degeneration Antibodies  >20 publications with UCHL1 and Nerve Degeneration
 Brain Injuries Antibodies  >17 publications with UCHL1 and Brain Injuries

Organs/Tissues associated with anti-UCHL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >128 publications with UCHL1 and Brain
 Testis Antibodies  >28 publications with UCHL1 and Testis
 Embryonic Tissue Antibodies  >20 publications with UCHL1 and Embryonic Tissue
 Liver Antibodies  >18 publications with UCHL1 and Liver
 Artery Antibodies  >16 publications with UCHL1 and Artery
 Eye Antibodies  >14 publications with UCHL1 and Eye
 Prostate Antibodies  >11 publications with UCHL1 and Prostate
 Pancreas Antibodies  >10 publications with UCHL1 and Pancreas
 Dorsal Root Ganglion Antibodies  >9 publications with UCHL1 and Dorsal Root Ganglion
 Ovary Antibodies  >3 publications with UCHL1 and Ovary
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