Rabbit Anti-MYO1F Polyclonal Antibody – Cat# MBS2003386

Western Blot: Sample: Recombinant protein.

Myosin if (MYO1F) Antibody – Rabbit Polyclonal

Name: MYO1F Antibody; Polyclonal; Rabbit; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS2003386
Price: 215 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2003386

MBS2003386 is an antibody targeting MYO1F. Also known as Polyclonal Antibody to Myosin IF (MYO1F). Reactive in Human, Mouse, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Myosin IF (MYO1F), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Myosin IF (MYO1F)

Product Gene Name

anti-MYO1F antibody

Immunogen Sequence

Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-EHFNSW SAGF VIHHYAGKVS YDVSGFCERN RDVLFSDLIE LMQTSEQAFL RMLFPEKLDG DKKGRPSTAG SKIKKQANDL VATLMRCTPH YIRCIKPNET KRPRDWEENR VKHQVEYLGL KENIRVRRAG FAYRRQFAKF LQRYAILTPE TWPRWRGDER QGVQHLLRAV NMEPDQYQMG STKVFVKNPE SLFLLEEVRE RKFDGFARTI QKAWRRHVAV RKYEEMREEA SNILLNKKER RRNSINRNFV GDYLGLEERP ELRQFLGKRE RVDFADS

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Form/Format

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.

Purity/Purification

Affinity Chromatography

Concentration

1mg/ml (lot specific)

Specificity

The antibody is a rabbit polyclonal antibody raised against MYO1F. It has been selected for its ability to recognize MYO1F in immunohistochemical staining andwestern blotting.

Preparation and Storage

Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Matching Pairs

Unconjugated Antibody: MYO1F (MBS2003386)
Immunogen: MYO1F (MBS2009955)

Matching Pairs

Unconjugated Antibody: MYO1F (MBS2003386)
APC-CY7 Conjugated Antibody: MYO1F (MBS2071507)

Molecular Weight

124,844 Da

OMIM

AJ310570 mRNA

3D Structure

ModBase 3D Structure for O00160

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Immunogen

Recombinant MYO1F (Glu491~Ser767) expressed in E.coli.

Cross Reactivity

Human

Conjugated Antibody

The APC conjugated antibody version of this item is also available as catalog #MBS2071509

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Applications Tested/Suitable for anti-MYO1F antibody

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

Application Notes for anti-MYO1F antibody

Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Other Notes

Small volumes of anti-MYO1F antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MYO1F. It may not necessarily be applicable to this product.

NCBI GI #

27544941

NCBI GeneID

4542

NCBI Accession #

NP_036467.2

UniProt Primary Accession #

O00160

UniProt Secondary Accession #

Q8WWN7

UniProt Related Accession #

O00160

GenBank Nucleotide #

NM_012335.3

NCBI Official Full Name

unconventional myosin-If isoform 1

NCBI Official Synonym Full Names

myosin IF

NCBI Official Symbol

MYO1F

UniProt Protein Name

Unconventional myosin-If

UniProt Synonym Protein Names

Myosin-Ie

Protein Family

Unconventional myosin

UniProt Gene Name

MYO1F

NCBI Protein Information

unconventional myosin-If

NCBI Summary for MYO1F

Myosins are molecular motors that use the energy from ATP hydrolysis to generate force on actin filaments. The protein encoded by this gene is an unconventional myosin that may be involved in the intracellular movement of membrane-enclosed compartments. There is evidence to suggest that mutations in this gene can result in hearing loss. [provided by RefSeq, Jan 2017]

UniProt Comments for MYO1F

MYO1F: Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: unconventional myosin complex

Molecular Function: actin binding; ATP binding; calmodulin binding; motor activity

Research Articles on MYO1F

1. identification of 6 heterozygous missense mutations in MYO1C and additional 5 heterozygous missense mutations in MYO1F in bilateral sensorineural hearing loss

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-MYO1F antibody

Disease Name	Pubmed Publications
Leukemia, Myeloid Antibodies	>2 publications with MYO1F and Leukemia, Myeloid
Translocation, Genetic Antibodies	>2 publications with MYO1F and Translocation, Genetic
Leukemia, Myeloid, Acute Antibodies	>2 publications with MYO1F and Leukemia, Myeloid, Acute
Neoplasms Antibodies	>2 publications with MYO1F and Neoplasms

Organs/Tissues associated with anti-MYO1F antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>2 publications with MYO1F and Blood
Kidney Antibodies	>1 publications with MYO1F and Kidney