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CKB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200425

CKB (Creatine kinase B-type) is a cytoplasmic enzyme involved in energy homeostasis. The protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other… More details

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Product Overview

Online Datasheet in PDF
Product Name
CKB, Monoclonal Antibody
Full Product Name
CKB antibody
Product Gene Name
Product Synonym Names
Creatine kinase B-type; B-CK; CKBB; Creatine kinase B-type; BCK
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT8E4
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
42,644 Da
OMIM
M16451 mRNA
3D Structure
ModBase 3D Structure for P12277
Antigen Species
Human
Immunogen
Recombinant human CKB (1-381aa) purified from E. coli
Clone Note
Anti-human CKB mAb, clone AT8E4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CKB protein.

Product Categories/Family for anti-CKB antibody

Applications Tested/Suitable for anti-CKB antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-CKB antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: 293T cell lysate Lane 2.: K562 cell lysate Lane 2.: LnCap cell lysate
Mouse Anti-CKB Monoclonal Antibody – Cat# MBS200425

Immunofluorescence (IF) of anti-CKB antibody

ICC/IF analysis of CKB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-CKB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200425

Product References and Citations for anti-CKB antibody

Chang EJ., et al. (2008) Nat Med. 14 (9): 966-72. ; Debrincat MA., et al. (2007) J Biol Chem. 282 (7): 4728-37.;

Other Notes

Small volumes of anti-CKB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CKB purchase

MBS200425 is an antibody targeting CKB. Also known as CKB antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CKB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
creatine kinase B-type
NCBI Official Synonym Full Names
creatine kinase B
NCBI Official Symbol
CKB
NCBI Official Synonym Symbols
BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29
UniProt Protein Name
Creatine kinase B-type
UniProt Synonym Protein Names
Brain creatine kinase
Protein Family
UniProt Gene Name
CKB
UniProt Synonym Gene Names
CKBB; CPK-B
NCBI Protein Information
creatine kinase B-type

NCBI Summary for CKB

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008]
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UniProt Comments for CKB

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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