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CALB2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200441

CALB2 is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CALB2, Monoclonal Antibody
Full Product Name
CALB2 antibody
Product Gene Name
Product Synonym Names
Calretinin; CALB2; CAL2; CR; 29 kDa calbindin; Calretinin
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT5C5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
31,540 Da
OMIM
X56667 mRNA
Antigen Species
Human
Immunogen
Recombinant human CALB2 (1-271aa) purified from E. coli
Clone Note
Anti-human CALB2 mAb, clone AT5C5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CALB2 protein.

Product Categories/Family for anti-CALB2 antibody

Applications Tested/Suitable for anti-CALB2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-CALB2 antibody

The cell and tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CALB2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse brain lysate
Mouse Anti-CALB2 Monoclonal Antibody – Cat# MBS200441

Immunofluorescence (IF) of anti-CALB2 antibody

ICC/IF analysis of CALB2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CALB2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-CALB2 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200441

Product References and Citations for anti-CALB2 antibody

Rogers JH., et al. (1987) J Cell Biol. 105 (3): 1343-1353.; Dreher B.,et al. (1996) J. Comp. Neurol. 376 (2): 223-40.

Other Notes

Small volumes of anti-CALB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CALB2 purchase

MBS200441 is an antibody targeting CALB2. Also known as CALB2 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CALB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Calbindin 2
NCBI Official Synonym Full Names
calbindin 2
NCBI Official Symbol
CALB2
NCBI Official Synonym Symbols
CR; CAL2; CAB29
UniProt Protein Name
Calretinin
UniProt Synonym Protein Names
29 kDa calbindin
Protein Family
UniProt Gene Name
CALB2
UniProt Synonym Gene Names
CAB29; CR
NCBI Protein Information
calretinin

NCBI Summary for CALB2

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
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UniProt Comments for CALB2

Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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