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GOT1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200451

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and use the urea and tricarboxylic acid cycles. The… More details

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Product Overview

Online Datasheet in PDF
Product Name
GOT1, Monoclonal Antibody
Full Product Name
GOT1 antibody
Product Gene Name
anti-GOT1 antibody
Product Synonym Names
Glutamate oxaloacetate transaminase 1 soluble; cAspAT
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT3G11
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C.
Avoid repeated freezing and thawing cycles.
OMIM
M37400 mRNA
3D Structure
ModBase 3D Structure for P17174
Immunogen
Recombinant human GOT1 (1-413aa) purified from E. coli

Product Categories/Family for anti-GOT1 antibody

Signal Transduction; Metabolism
Applications Tested/Suitable for anti-GOT1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-GOT1 antibody
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity.
Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Western Blot (WB) of anti-GOT1 antibody

The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : A427 cell lysate
Mouse Anti-GOT1 Monoclonal Antibody – Cat# MBS200451

Western Blot (WB) of anti-GOT1 antibody

The recombinat proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : Recombinant human GOT1
Lane 2 : Recombinant human GOT2
Mouse Anti-GOT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS200451

Western Blot (WB) of anti-GOT1 antibody

The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Lane 1 : 293T cell lysate
Lane 2 : GOT1 Transfected 293T cell lysate
Mouse Anti-GOT1 Monoclonal Antibody, Western Blot (WB) – Cat# MBS200451

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-GOT1 antibody

ICC/IF analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-GOT1 Monoclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS200451

Immunocytochemistry/Immunofluorescence (ICC/IF) of anti-GOT1 antibody

ICC/IF analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Mouse Anti-GOT1 Monoclonal Antibody, Immunocytochemistry/Immunofluorescence (ICC/IF) – Cat# MBS200451

Flow Cytometry (FC/FACS) of anti-GOT1 antibody

Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Mouse Anti-GOT1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS200451

Flow Cytometry (FC/FACS) of anti-GOT1 antibody

Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
Mouse Anti-GOT1 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS200451

Product References and Citations for anti-GOT1 antibody

Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22): 5293-9. ; Totan A., et al. (2006) Clin Chem Lab Med. 44 (5): 612-5.

Other Notes

Small volumes of anti-GOT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GOT1 purchase

MBS200451 is an antibody targeting GOT1. Also known as GOT1 antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for GOT1. It may not necessarily be applicable to this product.

NCBI GI #
4504067
NCBI GeneID
2805
NCBI Accession #
NP_002070
UniProt Primary Accession #
P17174
UniProt Secondary Accession #
Q5VW80; B2R6R7; B7Z7E9
UniProt Related Accession #
P17174
GenBank Nucleotide #
NM_002079.2
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1
NCBI Official Symbol
GOT1
NCBI Official Synonym Symbols
AST1; cCAT; GIG18; cAspAT; ASTQTL1
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family
Aspartate aminotransferase
UniProt Gene Name
GOT1
UniProt Synonym Gene Names
cAspAT; cCAT
NCBI Protein Information
aspartate aminotransferase, cytoplasmic

NCBI Summary for GOT1

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
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UniProt Comments for GOT1

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
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Research Articles on GOT1

1. High AST1 expression is associated with high mortality in hemodialysis patients.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GOT1 antibody

 Reference Product  PubMed Publications
 MDH1 antibody  >5 publications with GOT1 and MDH1
 GLUD1 antibody  >4 publications with GOT1 and GLUD1
 GPT2 antibody  >4 publications with GOT1 and GPT2
 LDHB antibody  >3 publications with GOT1 and LDHB
 MDH2 antibody  >2 publications with GOT1 and MDH2

Pathways associated with anti-GOT1 antibody

 Products by Pathway  Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 2-Oxocarboxylic Acid Metabolism Pathway antibodies  2-Oxocarboxylic Acid Metabolism Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Amino Acid Metabolism Pathway antibodies  Amino Acid Metabolism Pathway Diagram
 Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies  Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine And Proline Metabolism Pathway antibodies  Arginine And Proline Metabolism Pathway Diagram
 Arginine Biosynthesis Pathway antibodies  Arginine Biosynthesis Pathway Diagram

Diseases associated with anti-GOT1 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >8 publications with GOT1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >8 publications with GOT1 and Nervous System Diseases
 Liver Diseases Antibodies  >6 publications with GOT1 and Liver Diseases
 Lung Neoplasms Antibodies  >6 publications with GOT1 and Lung Neoplasms
 Brain Diseases Antibodies  >5 publications with GOT1 and Brain Diseases
 Fatty Liver Antibodies  >4 publications with GOT1 and Fatty Liver
 Cardiovascular Diseases Antibodies  >4 publications with GOT1 and Cardiovascular Diseases
 Fibrosis Antibodies  >3 publications with GOT1 and Fibrosis
 Liver Neoplasms Antibodies  >3 publications with GOT1 and Liver Neoplasms
 Heart Diseases Antibodies  >2 publications with GOT1 and Heart Diseases

Organs/Tissues associated with anti-GOT1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >22 publications with GOT1 and Blood
 Liver Antibodies  >13 publications with GOT1 and Liver
 Intestine Antibodies  >10 publications with GOT1 and Intestine
 Lung Antibodies  >7 publications with GOT1 and Lung
 Nerve Antibodies  >7 publications with GOT1 and Nerve
 Brain Antibodies  >7 publications with GOT1 and Brain
 Heart Antibodies  >5 publications with GOT1 and Heart
 Muscle Antibodies  >4 publications with GOT1 and Muscle
 Vascular Antibodies  >3 publications with GOT1 and Vascular
 Thyroid Antibodies  >2 publications with GOT1 and Thyroid
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