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ACTB Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS200463

Beta-actin (human gene and protein symbol ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility,… More details

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Product Overview

Product Name
ACTB, Monoclonal Antibody
Full Product Name
ACTB antibody
Product Gene Name
anti-ACTB antibody
Product Synonym Names
Actin; cytoplasmic 1; Beta-actin
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT4G6
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Purity/Purification
By protein-A affinity chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
41,737 Da
OMIM
X00351 mRNA
3D Structure
ModBase 3D Structure for P60709
Antigen Species
Human
Immunogen
Recombinant human ACTB (1-375aa) purified from E. coli.
Clone Note
Anti-human ACTB mAb, clone AT4G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACTB protein.

Product Categories/Family for anti-ACTB antibody

Cytoskeleton
Applications Tested/Suitable for anti-ACTB antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Western Blot (WB) of anti-ACTB antibody

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HeLa cell lysate Lane 3.: Jurkat cell lysate Lane 4.: PC3 cell lysate Lane 5.: A431 cell lysate Lane 6.: HepG2 cell lysate Lane 7.: MCF7 cell lysate Lane 8.: U87-MG cell lysate Lane 9.: NIH-3T3 cell lysate The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Brain tissue lysate Lane 2.: Kidney tissue lysate Lane 3.: Liver tissue lysate Lane 4.: Lung tissue lysate Lane 5.: Spleen tissue lysate Lane 6.: Testis tissue lysate Lane 7.: Bone marrow tissue lysate Lane 8.: Stomach tissue lysate The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: mouse monoclonal ACTB antibody 1:1000 Lane 2.: mouse monoclonal ACTB antibody 1:3000 Lane 3.: mouse monoclonal ACTB antibody 1:5000 Lane 4.: mouse monoclonal ACTB antibody 1:10000
Mouse Anti-ACTB Monoclonal Antibody – Cat# MBS200463

Immunofluorescence (IF) of anti-ACTB antibody

ICC/IF analysis of ACTB in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ACTB antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Mouse Anti-ACTB Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS200463

Product References and Citations for anti-ACTB antibody

Gunning PW., et al. (2015) Journal of Cell Science. 128 (11): 2009-2019.; Hanukoglu I., et al. (1983) Journal of Molecular Biology. 163 (4): 673-8.

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS200463 is an antibody targeting ACTB. Also known as ACTB antibody. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
4501885
NCBI GeneID
60
NCBI Accession #
NP_001092
UniProt Primary Accession #
P60709
UniProt Secondary Accession #
P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
UniProt Related Accession #
P60709
GenBank Nucleotide #
NM_001101.4
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
Actin
UniProt Gene Name
ACTB
NCBI Protein Information
actin, cytoplasmic 1

NCBI Summary for ACTB

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
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UniProt Comments for ACTB

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Research Articles on ACTB

1. The authors describe heterozygous ACTB deletions and nonsense and frameshift mutations in 33 individuals with developmental delay, apparent intellectual disability, increased frequency of internal organ malformations (including those of the heart and the renal tract), growth retardation, and a recognizable facial gestalt.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACTB antibody

 Reference Product  PubMed Publications
 ACTG1 antibody  >23 publications with ACTB and ACTG1
 CFL1 antibody  >2 publications with ACTB and CFL1
 PFN1 antibody  >1 publications with ACTB and PFN1

Pathways associated with anti-ACTB antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies  B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram

Diseases associated with anti-ACTB antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >68 publications with ACTB and Carcinoma
 Disease Models, Animal Antibodies  >28 publications with ACTB and Disease Models, Animal
 Inflammation Antibodies  >28 publications with ACTB and Inflammation
 Liver Diseases Antibodies  >26 publications with ACTB and Liver Diseases
 Lung Diseases Antibodies  >23 publications with ACTB and Lung Diseases
 Necrosis Antibodies  >23 publications with ACTB and Necrosis
 Kidney Diseases Antibodies  >19 publications with ACTB and Kidney Diseases
 Lung Neoplasms Antibodies  >19 publications with ACTB and Lung Neoplasms
 Heart Diseases Antibodies  >17 publications with ACTB and Heart Diseases
 Hypoxia Antibodies  >16 publications with ACTB and Hypoxia

Organs/Tissues associated with anti-ACTB antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >110 publications with ACTB and Blood
 Brain Antibodies  >87 publications with ACTB and Brain
 Muscle Antibodies  >70 publications with ACTB and Muscle
 Liver Antibodies  >66 publications with ACTB and Liver
 Kidney Antibodies  >45 publications with ACTB and Kidney
 Lung Antibodies  >42 publications with ACTB and Lung
 Bone Antibodies  >41 publications with ACTB and Bone
 Heart Antibodies  >40 publications with ACTB and Heart
 Connective Tissue Antibodies  >31 publications with ACTB and Connective Tissue
 Vascular Antibodies  >27 publications with ACTB and Vascular
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