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Defensin Alpha 3, Neutrophil Specific (DEFa3) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2006635
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MBS2006635 is an antibody targeting DEFa3. Also known as Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3). Reactive in Human. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Defensin Alpha 3, Neutrophil Specific (DEFa3), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Defensin Alpha 3, Neutrophil Specific (DEFa3)
Product Gene Name
anti-DEFa3 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
0.25mg/mL (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: DEFa3 (MBS2006635)
Biotin Conjugated Antibody: DEFa3 (MBS2004715)
Matching Pairs
Unconjugated Antibody: DEFa3 (MBS2006635)
Immunogen: DEFa3 (MBS2010173)
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OMIM
M21131 mRNA
3D Structure
ModBase 3D Structure for P59666
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant DEFa3 (Pro21~Cys94) expressed in E.coli (MBS2010173).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-DEFa3 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-DEFa3 antibody
Western blotting: 0.01-2ug/mL
I mmunohistochemistry: 5-30ug/mL
I mmunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-DEFa3 antibody

Western Blot:
Sample: Recombinant DEFa3, Human.
Rabbit Anti-DEFa3 Polyclonal Antibody – Cat# MBS2006635

Immunohistochemistry (IHC) of anti-DEFa3 antibody

DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-DEFa3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006635

Immunohistochemistry (IHC) of anti-DEFa3 antibody

DAB staining on IHC-P;
Samples: Human Pancreas Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-DEFa3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006635

Immunohistochemistry (IHC) of anti-DEFa3 antibody

DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody;
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-DEFa3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006635

Immunohistochemistry (IHC) of anti-DEFa3 antibody

DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-DEFa3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006635

Immunohistochemistry (IHC) of anti-DEFa3 antibody

DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-DEFa3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006635

Immunohistochemistry (IHC) of anti-DEFa3 antibody

DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 30ug/ml Rabbit Anti-Human DEFa3 Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Rabbit Anti-DEFa3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006635

Other Notes

Small volumes of anti-DEFa3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DEFa3. It may not necessarily be applicable to this product.

NCBI GI #
4885179
NCBI GeneID
1668
NCBI Accession #
NP_005208.1
UniProt Primary Accession #
P59666
UniProt Secondary Accession #
P11479; Q14125
UniProt Related Accession #
P59666
GenBank Nucleotide #
NM_005217.3
NCBI Official Full Name
neutrophil defensin 3 preproprotein
NCBI Official Synonym Full Names
defensin alpha 3
NCBI Official Symbol
DEFA3
NCBI Official Synonym Symbols
HP3; DEF3; HNP3; HP-3; HNP-3
UniProt Protein Name
Neutrophil defensin 3
UniProt Synonym Protein Names
Defensin, alpha 3; HNP-3; HP-3; HP3
Protein Family
Alpha-defensin
UniProt Gene Name
DEFA3
UniProt Synonym Gene Names
DEF3; HP-3; HP3; HP-2; HP2
NCBI Protein Information
neutrophil defensin 3

NCBI Summary for DEFa3

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation. [provided by RefSeq, Oct 2014]
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UniProt Comments for DEFa3

defensin, alpha 3: Defensin 2 and defensin 3 have antibiotic, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space; Golgi lumen

Molecular Function: protein homodimerization activity

Biological Process: antibacterial humoral response; antimicrobial humoral response; defense response to fungus; defense response to Gram-positive bacterium; defense response to virus; innate immune response in mucosa; intracellular estrogen receptor signaling pathway; killing of cells of other organism
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Research Articles on DEFa3

1. these results demonstrate that HNPs1-3 may be potent inhibitors of ADAMTS13 activity, likely by binding to the central A2 domain of VWF and physically blocking ADAMTS13 binding.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DEFa3 antibody

 Reference Product  PubMed Publications
 DEFA1 antibody  >15 publications with DEFa3 and DEFA1
 DEFA4 antibody  >3 publications with DEFa3 and DEFA4
 DEFB1 antibody  >2 publications with DEFa3 and DEFB1
 CAMP antibody  >1 publications with DEFa3 and CAMP
 DEFA5 antibody  >1 publications with DEFa3 and DEFA5
 DEFA6 antibody  >1 publications with DEFa3 and DEFA6

Pathways associated with anti-DEFa3 antibody

 Products by Pathway  Pathway Diagram
 Alpha-defensins Pathway antibodies  Alpha-defensins Pathway Diagram
 Antimicrobial Peptides Pathway antibodies  Antimicrobial Peptides Pathway Diagram
 Defensins Pathway antibodies  Defensins Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Innate Immune System Pathway antibodies  Innate Immune System Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram

Diseases associated with anti-DEFa3 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >7 publications with DEFa3 and Inflammation
 Nervous System Diseases Antibodies  >2 publications with DEFa3 and Nervous System Diseases
 Skin Diseases Antibodies  >1 publications with DEFa3 and Skin Diseases
 Glioma Antibodies  >1 publications with DEFa3 and Glioma
 Necrosis Antibodies  >1 publications with DEFa3 and Necrosis

Organs/Tissues associated with anti-DEFa3 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >16 publications with DEFa3 and Blood
 Connective Tissue Antibodies  >1 publications with DEFa3 and Connective Tissue
 Kidney Antibodies  >1 publications with DEFa3 and Kidney
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