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Profilin 1 (PFN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2006947
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MBS2006947 is an antibody targeting PFN1. Also known as Polyclonal Antibody to Profilin 1 (PFN1). Reactive in Mouse, Human, Rat. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Profilin 1 (PFN1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Profilin 1 (PFN1)
Product Gene Name
anti-PFN1 antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -AGWNAYIDS LMADGTCQDA AIVGYKDSPS VWAAVPGKTF VSITPAEVGV LVGKDRSSFF VNGLTLGGQK CSVIRDSLLQ DGEFTMDLRT KSTGGAPTFN VTVTMTAKTL VLLMGKEGVH GGLINKKCYE MASHLRRSQY
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Human, Rat
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against PFN1. It has been selected for its ability to recognize PFN1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: PFN1 (MBS2006947)
Biotin Conjugated Antibody: PFN1 (MBS2001913)
Matching Pairs
Unconjugated Antibody: PFN1 (MBS2006947)
Immunogen: PFN1 (MBS2009693)
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Molecular Weight
14,957 Da
3D Structure
ModBase 3D Structure for P62962
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant PFN1 (Ala2~Tyr140) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064152
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PFN1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-PFN1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-PFN1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-PFN1 Polyclonal Antibody – Cat# MBS2006947

Immunohistochemistry (IHC) of anti-PFN1 antibody

DABstainingonIHC-P.Samples:MouseTissue)
Rabbit Anti-PFN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2006947

Other Notes

Small volumes of anti-PFN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PFN1. It may not necessarily be applicable to this product.

NCBI GI #
6755040
NCBI GeneID
18643
NCBI Accession #
NP_035202.1
UniProt Primary Accession #
P62962
UniProt Secondary Accession #
P10924
UniProt Related Accession #
P62962
GenBank Nucleotide #
NM_011072.4
NCBI Official Full Name
profilin-1
NCBI Official Synonym Full Names
profilin 1
NCBI Official Symbol
Pfn1
NCBI Official Synonym Symbols
Pfn
UniProt Protein Name
Profilin-1
UniProt Synonym Protein Names
Profilin I
UniProt Gene Name
Pfn1
NCBI Protein Information
profilin-1

UniProt Comments for PFN1

profilin 1: a ubiquitous actin monomer-binding protein belonging to the profilin family. At high concentrations, profilin 1 prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Deletion of this gene is associated with Miller-Dieker syndrome.

Protein type: Actin-binding; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11 B3|11 43.21 cM

Cellular Component: blood microparticle; cell cortex; cytoplasm; cytoskeleton; extracellular exosome; extracellular space; focal adhesion; membrane; neuron projection; nucleus; synapse

Molecular Function: actin binding; actin monomer binding; adenyl-nucleotide exchange factor activity; cadherin binding; phosphatidylinositol-4,5-bisphosphate binding; proline-rich region binding; protein binding; receptor binding; Rho GTPase binding; RNA binding

Biological Process: actin cytoskeleton organization; negative regulation of actin filament bundle formation; negative regulation of actin filament polymerization; negative regulation of stress fiber formation; neural tube closure; positive regulation of actin filament bundle assembly; positive regulation of actin filament polymerization; positive regulation of ATPase activity; positive regulation of DNA metabolic process; positive regulation of epithelial cell migration; positive regulation of ruffle assembly; positive regulation of stress fiber formation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of viral transcription; protein stabilization; regulation of actin polymerization and/or depolymerization; regulation of transcription from RNA polymerase II promoter; sequestering of actin monomers
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Research Articles on PFN1

1. Profilin1 is expressed in osteocytes and regulates cell shape, migration and bone mass.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-PFN1 antibody

 Reference Product  PubMed Publications
 Enah antibody  >5 publications with PFN1 and Enah

Pathways associated with anti-PFN1 antibody

 Products by Pathway  Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 G13 Signaling Pathway antibodies  G13 Signaling Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Regulation Of Actin Cytoskeleton Pathway antibodies  Regulation Of Actin Cytoskeleton Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Signaling By Robo Receptor Pathway antibodies  Signaling By Robo Receptor Pathway Diagram

Diseases associated with anti-PFN1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >21 publications with PFN1 and Neoplasms
 Breast Neoplasms Antibodies  >9 publications with PFN1 and Breast Neoplasms
 Adenocarcinoma Antibodies  >8 publications with PFN1 and Adenocarcinoma
 Liver Neoplasms Antibodies  >4 publications with PFN1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >4 publications with PFN1 and Carcinoma, Hepatocellular
 Nervous System Diseases Antibodies  >4 publications with PFN1 and Nervous System Diseases
 Kidney Diseases Antibodies  >4 publications with PFN1 and Kidney Diseases
 Liver Diseases Antibodies  >4 publications with PFN1 and Liver Diseases
 Blood Coagulation Disorders Antibodies  >4 publications with PFN1 and Blood Coagulation Disorders
 Arteriosclerosis Antibodies  >3 publications with PFN1 and Arteriosclerosis

Organs/Tissues associated with anti-PFN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >36 publications with PFN1 and Blood
 Brain Antibodies  >28 publications with PFN1 and Brain
 Muscle Antibodies  >20 publications with PFN1 and Muscle
 Kidney Antibodies  >11 publications with PFN1 and Kidney
 Embryonic Tissue Antibodies  >10 publications with PFN1 and Embryonic Tissue
 Bone Antibodies  >7 publications with PFN1 and Bone
 Liver Antibodies  >7 publications with PFN1 and Liver
 Bone Marrow Antibodies  >6 publications with PFN1 and Bone Marrow
 Spleen Antibodies  >6 publications with PFN1 and Spleen
 Thymus Antibodies  >5 publications with PFN1 and Thymus
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