3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Carboxylesterase 1/CES1 (CES1) Active Protein

Name: CES1 Active Protein; Carboxylesterase 1/CES1; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS201130
Price: 290 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201130

Carboxylesterase 1/CES1, also known as liver carboxylesterase 1, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. CES1G shares the serine hydrolase fold observed in other esterases. It is a… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Carboxylesterase 1/CES1, Active Protein

Full Product Name

Recombinant Carboxylesterase 1/CES1

Product Gene Name

CES1 active protein

Product Synonym Names

Liver carboxylesterase 1; Carboxylesterase 1/CES1; CES1G; Ces-1; Ces1; Ses-1; Acyl-coenzyme A:cholesterol acyltransferase; Carboxylesterase 1G; ES-x

Sequence

ADP HPSLPPV VHTVHGKVLG KYVTLEGFSQ PVAVFLGVPF AKPPLGSLRF APPEPAEPWS FVKHTTSYPP LCYQNPEAAL RLAELFTNQR KIIPHKFSED CLYLNIYTPA DLTQNSRLPV MVWIHGGGLV IDGASTYDGV PLAVHENVVV VVIQYRLGIW GFFSTEDEHS RGNWGHLDQV AALHWVQDNI ANFGGNPGSV TIFGESAGGE SVSVLVLSPL AKNLFHRAIA QSSVIFNPCL FGRAARPLAK KIAALAGCKT TTSAAMVHCL RQKTEDELLE VSLKMKFGTV DFLGDPRESY PFLPTVIDGV LLPKAPEEIL AEKSFNTVPY MVGINKHEFG WIIPMFLDFP LSERKLDQKT AASILWQAYP ILNISEKLIP AAIEKYLGGT EDPATMTDLF LDLIGDIMFG VPSVIVSRSH RDAGAPTYMY EYQYRPSFVS DDRPQELLGD HADELFSVWG APFLKEGASE EEINLSKMVM KFWANFARNG NPNGEGLPHW PEYDQKEGYL QIGVPAQAAH RLKDKEVDFW TELRAKETAE RSSHREHVEL HHHHHH

Host

Baculovirus

Form/Format

Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.

Purity/Purification

>90% by SDS-PAGE

Concentration

0.5mg/ml (determined by Absorbance at 280nm) (lot specific)

Preparation and Storage

Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

Molecular Weight

61.9kDa (556aa); 50-70kDa (SDS-PAGE under reducing conditions)

Sequence Positions

19-565aa

3D Structure

ModBase 3D Structure for Q8VCC2

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Species

Mouse

Endotoxin

<1 EU per 1ug of protein (determined by LAL method)

Tag

His-Tag

Predicted N-Terminal

Ala

Biological Activity

Specific activity is > 2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C.

Product Categories/Family for CES1 active protein

Cardiovascular

Applications Tested/Suitable for CES1 active protein

SDS-PAGE, Enzyme Activity

Product References and Citations for CES1 active protein

Satoh T., et al. (2006) Chem Biol Interact. 162:195-211. Fleming CD., et al. (2007) Biochemistry. 46:5063-5071.

Other Notes

Small volumes of CES1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CES1 purchase

MBS201130 is an active protein targeting CES1. Also known as Recombinant Carboxylesterase 1/CES1. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for CES1. It may not necessarily be applicable to this product.

NCBI GI #

162287349

NCBI Accession #

NP_067431.2

UniProt Primary Accession #

Q8VCC2

GenBank Nucleotide #

NP_067431.2

NCBI Official Full Name

liver carboxylesterase 1

UniProt Protein Name

Liver carboxylesterase 1

UniProt Synonym Protein Names

Acyl-coenzyme A:cholesterol acyltransferase; Carboxylesterase 1G; ES-x

Protein Family

Liver carboxylesterase

UniProt Gene Name

Ces1

UniProt Synonym Gene Names

Ces1g

UniProt Comments for CES1

CES1: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.1; EC 3.1.1.56; Hydrolase; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 8 C5|8 45.29 cM

Cellular Component: extracellular space

Biological Process: regulation of gene expression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with CES1 active protein

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Phase 1 - Functionalization Of Compounds Pathway antibodies	Phase 1 - Functionalization Of Compounds Pathway Diagram
Phase I, Non P450 Pathway antibodies	Phase I, Non P450 Pathway Diagram

Diseases associated with CES1 active protein

Disease Name	Pubmed Publications
Fatty Liver Antibodies	>1 publications with CES1 and Fatty Liver

Organs/Tissues associated with CES1 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>3 publications with CES1 and Liver