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Thrombopoietin (THPO) Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201194

Thrombopoietin(THPO), also known as megakaryocyte growth and development factor (MGDF), is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets. It stimulates the production and differentiation of megakaryocytes,… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Thrombopoietin (Thpo), Active Protein
Full Product Name
Thrombopoietin
Product Gene Name
Thpo active protein
Product Synonym Names
Thpo; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand; Mgdf; Ml; Mpllg; Tpo; thrombopoietin isoform 1
Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HGPVNGTHGL FAGTSLQTLE ASDISPGAFN KGSLAFNLQG GLPPSPSLAP DGHTPFPPSP ALPTTHGSPP QLHPLFPDPS TTMPNSTAPH PVTMYPHPRN LSQET
Host
HEK293
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
Purity/Purification
>90% by SDS - PAGE
Concentration
0.5mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
Molecular Weight
36.4kDa (341aa)
Sequence Positions
22-356aa
3D Structure
ModBase 3D Structure for P40226
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Mouse
Tag
His-Tag
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Predicted N-Terminal
Ser 22
Biological activity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range <=4 ng/ml.

Product Categories/Family for Thpo active protein

Cardiovascular
Applications Tested/Suitable for Thpo active protein
SDS-PAGE, Bioactivity

SDS-Page of Thpo active protein

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
THPO Active Protein – Cat# MBS201194

Product References and Citations for Thpo active protein

Jenkins JM., et al. (2007) Blood. 109:4739-4741; Hitchcock IS., et al. (2014) Br J Haemato. 165:259-268.

Other Notes

Small volumes of Thpo active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Thpo purchase

MBS201194 is an active protein targeting Thpo. Also known as Thrombopoietin. Host: HEK293.

Additional Details

NCBI/Uniprot data below describe general gene information for Thpo. It may not necessarily be applicable to this product.

NCBI GI #
6678341
NCBI GeneID
21832
NCBI Accession #
NP_033405
UniProt Primary Accession #
P40226
UniProt Related Accession #
P40226
GenBank Nucleotide #
NP_033405.1
NCBI Official Full Name
thrombopoietin isoform 1
NCBI Official Synonym Full Names
thrombopoietin
NCBI Official Symbol
Thpo
NCBI Official Synonym Symbols
Ml; Tpo; Mgdf; Mpllg
UniProt Protein Name
Thrombopoietin
UniProt Synonym Protein Names
C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Protein Family
Thrombopoietin
UniProt Gene Name
Thpo
UniProt Synonym Gene Names
ML; MGDF
UniProt Entry Name
TPO_MOUSE
NCBI Protein Information
thrombopoietin

NCBI Summary for Thpo

This gene encodes a humoral growth factor necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. The encoded protein is a ligand for the product of the myeloproliferative leukemia virus oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
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UniProt Comments for Thpo

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide; Cytokine

Cellular Component: extracellular space; extracellular region

Molecular Function: hormone activity; cytokine activity; receptor binding

Biological Process: cell proliferation; positive regulation of protein kinase B signaling cascade; positive regulation of megakaryocyte differentiation; myeloid cell differentiation; positive regulation of protein amino acid phosphorylation
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Research Articles on Thpo

1. Thpo-regulated mitochondrial activity associated with mitochondrial translocation of STAT3 phosphorylated at serine 727.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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