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ACAT2 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS201221

Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that inhumans is encoded by the ACAT (acetyl-Coenzyme A acetyltransferase 2) gene. ACAT2 is an acetyl-CoAC-acetyltransferase enzyme, plays a role in… More details

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Product Overview

Online Datasheet in PDF
Product Name
ACAT2, Monoclonal Antibody
Full Product Name
ACAT2
Product Gene Name
anti-ACAT2 antibody
Product Synonym Names
Acetyl-CoA acetyltransferase cytosolic; ACTL; Acetyl-CoA acetyltransferase; cytosolic
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT55D5
Host
Mouse
Species Reactivity
Human
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Purity/Purification
By protein-A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
OMIM
100678
3D Structure
ModBase 3D Structure for Q9BWD1
Antigen Species
Human
Immunogen
Recombinant human ACAT2 (1-397aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.

Product Categories/Family for anti-ACAT2 antibody

Cardiovascular; Metabolism; Signal Transduction
Applications Tested/Suitable for anti-ACAT2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ACAT2 antibody
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity.

Immunocytochemistry (ICC)/Immunofluorescence (IF) of anti-ACAT2 antibody

ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Mouse Anti-ACAT2 Monoclonal Antibody – Cat# MBS201221

Western Blot (WB) of anti-ACAT2 antibody

The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HepG2 cell lysate Lane 2.: Ramos cell lysate
Mouse Anti-ACAT2 Monoclonal Antibody, Western Blot (WB) – Cat# MBS201221

Product References and Citations for anti-ACAT2 antibody

Shintani S., et al. (1999) Genetics. 152(2): 743-754.; Middleton B., et al. (1974) Biochem J. 139(1): 109-121.

Other Notes

Small volumes of anti-ACAT2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACAT2 purchase

MBS201221 is an antibody targeting ACAT2. Reactive in Human. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for ACAT2. It may not necessarily be applicable to this product.

NCBI GI #
148539872
NCBI GeneID
39
NCBI Accession #
NP_005882
UniProt Primary Accession #
Q9BWD1
UniProt Related Accession #
Q9BWD1
GenBank Nucleotide #
NP_005882.2
NCBI Official Full Name
acetyl-CoA acetyltransferase, cytosolic isoform 1
NCBI Official Synonym Full Names
acetyl-CoA acetyltransferase 2
NCBI Official Symbol
ACAT2
UniProt Protein Name
Acetyl-CoA acetyltransferase, cytosolic
UniProt Synonym Protein Names
Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase
Protein Family
Acetyl-CoA acetyltransferase
UniProt Gene Name
ACAT2
UniProt Synonym Gene Names
ACTL
UniProt Entry Name
THIC_HUMAN
NCBI Protein Information
acetyl-CoA acetyltransferase, cytosolic

NCBI Summary for ACAT2

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
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UniProt Comments for ACAT2

ACAT2: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - butanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Acetyltransferase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: acetyl-CoA C-acetyltransferase activity

Biological Process: lipid metabolic process

Disease: Acetyl-coa Acetyltransferase-2 Deficiency
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Research Articles on ACAT2

1. results demonstrate that the low-level expression of human ACAT2 gene with specific CpG-hypomethylated promoter is regulated by the C/EBP transcription factors in monocytic cells, and imply that the lowly expressed ACAT2 catalyzes the synthesis of certain CE/SE that are assembled into lipoproteins for the secretion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ACAT2 antibody

 Reference Product  PubMed Publications
 ACADS antibody  >1 publications with ACAT2 and ACADS
 HMGCS2 antibody  >1 publications with ACAT2 and HMGCS2
 HMGCS1 antibody  >1 publications with ACAT2 and HMGCS1

Pathways associated with anti-ACAT2 antibody

 Products by Pathway  Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 C5 Isoprenoid Biosynthesis, Mevalonate Pathway antibodies  C5 Isoprenoid Biosynthesis, Mevalonate Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fat Digestion And Absorption Pathway antibodies  Fat Digestion And Absorption Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram
 Fatty Acid Degradation Pathway antibodies  Fatty Acid Degradation Pathway Diagram

Diseases associated with anti-ACAT2 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >28 publications with ACAT2 and Cardiovascular Diseases
 Fatty Liver Antibodies  >17 publications with ACAT2 and Fatty Liver
 Liver Diseases Antibodies  >13 publications with ACAT2 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >7 publications with ACAT2 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >7 publications with ACAT2 and Liver Neoplasms
 Disease Models, Animal Antibodies  >6 publications with ACAT2 and Disease Models, Animal
 Heart Diseases Antibodies  >5 publications with ACAT2 and Heart Diseases
 Lung Neoplasms Antibodies  >3 publications with ACAT2 and Lung Neoplasms
 Weight Gain Antibodies  >3 publications with ACAT2 and Weight Gain
 Kidney Diseases Antibodies  >2 publications with ACAT2 and Kidney Diseases

Organs/Tissues associated with anti-ACAT2 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >75 publications with ACAT2 and Liver
 Blood Antibodies  >41 publications with ACAT2 and Blood
 Intestine Antibodies  >38 publications with ACAT2 and Intestine
 Vascular Antibodies  >7 publications with ACAT2 and Vascular
 Heart Antibodies  >7 publications with ACAT2 and Heart
 Kidney Antibodies  >4 publications with ACAT2 and Kidney
 Brain Antibodies  >4 publications with ACAT2 and Brain
 Adrenal Gland Antibodies  >3 publications with ACAT2 and Adrenal Gland
 Ovary Antibodies  >3 publications with ACAT2 and Ovary
 Lung Antibodies  >3 publications with ACAT2 and Lung
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