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GRB2 Associated Binding Protein 1 (GAB1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2013192
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MBS2013192 is an antibody targeting GAB1. Also known as Polyclonal Antibody to GRB2 Associated Binding Protein 1 (GAB1). Reactive in Human, Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
GRB2 Associated Binding Protein 1 (GAB1), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to GRB2 Associated Binding Protein 1 (GAB1)
Product Gene Name
anti-GAB1 antibody
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PVETC GVPRTASDTD SSYCIPPPAG MTPSRSNTIS TVDLNKLRKD ASSQDCYDIP RTFPSDRSSS LEGFHSQYKI KSVLTAGGVS GEELDENYVP MNPNSPPRQH SGSFTEPIQE PNYVPMTPGT FDFSSFGMQV PPPAHMGFRS SPKTPPRRPV PVADCEPPPV DRNLKPDRKV KPAPLDIKPL SEWEELQAPV
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against GAB1. It has been selected for its ability to recognize GAB1 in immunohistochemical staining andwestern blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: GAB1 (MBS2013192)
Immunogen: GAB1 (MBS2018685)
Matching Pairs
Unconjugated Antibody: GAB1 (MBS2013192)
APC-CY7 Conjugated Antibody: GAB1 (MBS2082342)
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Molecular Weight
76,812 Da
3D Structure
ModBase 3D Structure for Q9QYY0
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunogen
Recombinant GAB1 (Pro356~His584) expressed in E.coli.
Cross Reactivity
Human, Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2082344
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-GAB1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-GAB1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-GAB1 antibody

Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse GAB1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-GAB1 Polyclonal Antibody – Cat# MBS2013192

Western Blot (WB) of anti-GAB1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-GAB1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2013192

Immunohistochemistry (IHC) of anti-GAB1 antibody

DAB staining on IHC-P. Samples: Mouse Tissue)
Rabbit Anti-GAB1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2013192

Other Notes

Small volumes of anti-GAB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GAB1. It may not necessarily be applicable to this product.

NCBI GI #
31542871
NCBI GeneID
14388
NCBI Accession #
NP_067331.2
UniProt Primary Accession #
Q9QYY0
UniProt Secondary Accession #
Q91VW7
UniProt Related Accession #
Q9QYY0
GenBank Nucleotide #
NM_021356.2
NCBI Official Full Name
GRB2-associated-binding protein 1 isoform 2
NCBI Official Synonym Full Names
growth factor receptor bound protein 2-associated protein 1
NCBI Official Symbol
Gab1
NCBI Official Synonym Symbols
AA408973; AW107238
UniProt Protein Name
GRB2-associated-binding protein 1
UniProt Synonym Protein Names
GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1
Protein Family
GPI transamidase component
UniProt Gene Name
Gab1
NCBI Protein Information
GRB2-associated-binding protein 1

UniProt Comments for GAB1

GAB1: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Belongs to the GAB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8|8 C2

Cellular Component: cytoplasm

Molecular Function: protein binding; signal transducer activity

Biological Process: actin cytoskeleton reorganization; activation of JNK activity; activation of MAPK activity; angiogenesis; epidermal growth factor receptor signaling pathway; epidermis development; heart development; interleukin-6-mediated signaling pathway; platelet-derived growth factor receptor signaling pathway; positive regulation of angiogenesis; positive regulation of cell migration by vascular endothelial growth factor signaling pathway; positive regulation of MAPK cascade; regulation of cell migration; response to oxidative stress; signal transduction; vascular endothelial growth factor signaling pathway
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Research Articles on GAB1

1. Data, including data from studies in transgenic/knockout mice, suggest expression of Gab1/Gab2 is up-regulated in activated macrophages in pulmonary fibrosis; both Gab1/Gab2 are recruited to Il4r, synergistically enhancing downstream signal amplification. (Gab1 = growth factor receptor bound protein 2-associated protein 1; Gab2 = growth factor receptor bound protein 2-associated protein 2; Il4r = interleukin-4 receptor)
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GAB1 antibody

 Reference Product  PubMed Publications
 Grb2 antibody  >208 publications with GAB1 and Grb2
 Src antibody  >105 publications with GAB1 and Src
 Met antibody  >104 publications with GAB1 and Met
 Ptpn11 antibody  >70 publications with GAB1 and Ptpn11
 Egfr antibody  >60 publications with GAB1 and Egfr
 Frs2 antibody  >12 publications with GAB1 and Frs2
 Shc1 antibody  >10 publications with GAB1 and Shc1
 Crkl antibody  >6 publications with GAB1 and Crkl
 Pik3r1 antibody  >1 publications with GAB1 and Pik3r1

Pathways associated with anti-GAB1 antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
 Downstream Signal Transduction Pathway antibodies  Downstream Signal Transduction Pathway Diagram
 Downstream Signaling Events Of B Cell Receptor (BCR) Pathway antibodies  Downstream Signaling Events Of B Cell Receptor (BCR) Pathway Diagram
 Downstream Signaling Of Activated FGFR1 Pathway antibodies  Downstream Signaling Of Activated FGFR1 Pathway Diagram
 Downstream Signaling Of Activated FGFR2 Pathway antibodies  Downstream Signaling Of Activated FGFR2 Pathway Diagram

Diseases associated with anti-GAB1 antibody

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >18 publications with GAB1 and Cardiovascular Diseases
 Lung Diseases Antibodies  >17 publications with GAB1 and Lung Diseases
 Adenocarcinoma Antibodies  >14 publications with GAB1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >14 publications with GAB1 and Breast Neoplasms
 Vascular Diseases Antibodies  >13 publications with GAB1 and Vascular Diseases
 Inflammation Antibodies  >13 publications with GAB1 and Inflammation
 Disease Models, Animal Antibodies  >12 publications with GAB1 and Disease Models, Animal
 Neoplasms, Experimental Antibodies  >12 publications with GAB1 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >9 publications with GAB1 and Nervous System Diseases
 Necrosis Antibodies  >8 publications with GAB1 and Necrosis

Organs/Tissues associated with anti-GAB1 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >29 publications with GAB1 and Lung
 Liver Antibodies  >20 publications with GAB1 and Liver
 Kidney Antibodies  >19 publications with GAB1 and Kidney
 Brain Antibodies  >19 publications with GAB1 and Brain
 Embryonic Tissue Antibodies  >16 publications with GAB1 and Embryonic Tissue
 Heart Antibodies  >14 publications with GAB1 and Heart
 Spleen Antibodies  >10 publications with GAB1 and Spleen
 Skin Antibodies  >9 publications with GAB1 and Skin
 Prostate Antibodies  >7 publications with GAB1 and Prostate
 Eye Antibodies  >6 publications with GAB1 and Eye
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