Centromere Protein E (CENPE) Antibody – Rabbit Polyclonal

Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]

CENPE: Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle. Belongs to the kinesin-like protein family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: chromosome; chromosome, centromeric region; cytosol; kinesin complex; kinetochore; kinetochore microtubule; membrane; microtubule; microtubule cytoskeleton; midbody; nucleus; outer kinetochore of condensed chromosome

Molecular Function: ATP binding; ATPase activity; kinetochore binding; microtubule binding; microtubule motor activity; protein binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; attachment of mitotic spindle microtubules to kinetochore; cell division; chromosome segregation; kinetochore assembly; metaphase plate congression; microtubule-based movement; mitotic cell cycle; mitotic chromosome movement towards spindle pole; mitotic metaphase plate congression; mitotic spindle organization and biogenesis; multicellular organism development; positive regulation of protein kinase activity; regulation of mitotic metaphase/anaphase transition; retrograde vesicle-mediated transport, Golgi to ER; sister chromatid cohesion

Disease: Microcephaly 13, Primary, Autosomal Recessive