HSP27 Recombinant Protein

Name: HSP27 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203034
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203034

Hsp27 (also known as the Estrogen-Regulated 24K protein, and hsp 28) is a member of the mammalian small heat shock protein family. Hsp27 is expressed constitutively in many tissues and its expression is increased to high levels after various types of stress… More details

$210 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Hsp27, Recombinant Protein

Full Product Name

Hsp27, 1-205aa, Human, E Coli

Product Gene Name

Hsp27 recombinant protein

Product Synonym Names

Heat shock 27 kDa protein; Hspb1; Hsp25; Hsp27; Hsp27; HspB1; Heat shock 27 kDa protein; HSP 27; Growth-related 25 kDa protein; P25; HSP25; Heat shock protein beta-1; Heat shock 27 kDa protein 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen regulated 24 kDa protein; at shock 25kDa protein 1; Heat shock 27kDa protein 1; Heat shock 28kDa protein 1; Heat Shock Protein 27; Heat shock protein beta 1; Heat Shock Protein27; HS.76067; Hsp 28; Hsp B1; Hsp28; HspB1; SRP27; Stress responsive protein 27.

Sequence

MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK

Host

E Coli

Form/Format

Liquid. In 20 mM Hepesl buffer (pH 7.5) containing 100 mM KCl, 1 mM DTT

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

22.7kDa (205aa), confirmed by MALDI-TOF.

OMIM

L39370 Genomic DNA

3D Structure

ModBase 3D Structure for P04792

Antigen Species

Human

Endotoxin Level

< 1.0 EU per 1 microgram of protein (determined by LAL method)

Product Categories/Family for Hsp27 recombinant protein

Heat Shock Proteins

Applications Tested/Suitable for Hsp27 recombinant protein

SDS-PAGE

Product References and Citations for Hsp27 recombinant protein

Ehrnsperger M.,et al. (1997) EMBO J. 16, 221-229; Butt E., et al. (2001) J. Biol. Chem. 276, 7108-7113;

Other Notes

Small volumes of Hsp27 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Hsp27 purchase

MBS203034 is a recombinant protein targeting Hsp27. Also known as Hsp27, 1-205aa, Human, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for Hsp27. It may not necessarily be applicable to this product.

NCBI GI #

4504517

NCBI GeneID

3315

NCBI Accession #

NP_001531

UniProt Primary Accession #

P04792

UniProt Secondary Accession #

Q6FI47; Q96C20; Q96EI7; Q9UC31; Q9UC34; Q9UC35; Q9UC36; B2R4N8

UniProt Related Accession #

P04792

GenBank Nucleotide #

NM_001540.4

NCBI Official Full Name

heat shock protein beta-1

NCBI Official Synonym Full Names

heat shock protein family B (small) member 1

NCBI Official Symbol

HSPB1

NCBI Official Synonym Symbols

CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

UniProt Protein Name

Heat shock protein beta-1

UniProt Synonym Protein Names

28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Protein Family

Heat shock protein

UniProt Gene Name

HSPB1

UniProt Synonym Gene Names

HSP27; HSP28; HspB1; HSP 27; SRP27

NCBI Protein Information

heat shock protein beta-1

NCBI Summary for Hsp27

This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. [provided by RefSeq, Aug 2017]

UniProt Comments for Hsp27

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742).

Research Articles on Hsp27

1. found knock down of HSPB1 further increased the proportion of apoptotic cells in hyperthermic treated melanoma cells when compared with either single agent alone, and both agents leaded to cell cycle arrest at G0/G1 or G2/M phases

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Hsp27 recombinant protein

Reference Product	PubMed Publications
MAPKAPK2 recombinant protein	>59 publications with Hsp27 and MAPKAPK2
HSPA1A recombinant protein	>29 publications with Hsp27 and HSPA1A
HSPA8 recombinant protein	>22 publications with Hsp27 and HSPA8
MAPK14 recombinant protein	>7 publications with Hsp27 and MAPK14
MAPKAPK5 recombinant protein	>5 publications with Hsp27 and MAPKAPK5
MAPKAPK3 recombinant protein	>4 publications with Hsp27 and MAPKAPK3
ESR1 recombinant protein	>2 publications with Hsp27 and ESR1
EIF4G1 recombinant protein	>1 publications with Hsp27 and EIF4G1

Pathways associated with Hsp27 recombinant protein

Products by Pathway	Pathway Diagram
ATM Signaling Network In Development And Disease Pathway antibodies	ATM Signaling Network In Development And Disease Pathway Diagram
AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway antibodies	AUF1 (hnRNP D0) Binds And Destabilizes MRNA Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway antibodies	Apoptosis-related Network Due To Altered Notch3 In Ovarian Cancer Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Epstein-Barr Virus Infection Pathway antibodies	Epstein-Barr Virus Infection Pathway Diagram
Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation antibodies	Fas Ligand (FasL) Pathway And Stress Induction Of Heat Shock Proteins (HSP) Regulation Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
MAPK Signaling Pathway antibodies	MAPK Signaling Pathway Diagram

Diseases associated with Hsp27 recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>114 publications with Hsp27 and Disease Models, Animal
Inflammation Antibodies	>85 publications with Hsp27 and Inflammation
Necrosis Antibodies	>83 publications with Hsp27 and Necrosis
Heart Diseases Antibodies	>66 publications with Hsp27 and Heart Diseases
Liver Diseases Antibodies	>51 publications with Hsp27 and Liver Diseases
Lung Neoplasms Antibodies	>43 publications with Hsp27 and Lung Neoplasms
Reperfusion Injury Antibodies	>42 publications with Hsp27 and Reperfusion Injury
Kidney Diseases Antibodies	>38 publications with Hsp27 and Kidney Diseases
Liver Neoplasms Antibodies	>33 publications with Hsp27 and Liver Neoplasms
Carcinoma, Squamous Cell Antibodies	>32 publications with Hsp27 and Carcinoma, Squamous Cell

Organs/Tissues associated with Hsp27 recombinant protein

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>263 publications with Hsp27 and Muscle
Blood Antibodies	>207 publications with Hsp27 and Blood
Brain Antibodies	>199 publications with Hsp27 and Brain
Heart Antibodies	>134 publications with Hsp27 and Heart
Nerve Antibodies	>124 publications with Hsp27 and Nerve
Vascular Antibodies	>115 publications with Hsp27 and Vascular
Lung Antibodies	>95 publications with Hsp27 and Lung
Liver Antibodies	>94 publications with Hsp27 and Liver
Kidney Antibodies	>81 publications with Hsp27 and Kidney
Eye Antibodies	>56 publications with Hsp27 and Eye