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Prenylcysteine Oxidase 1 (PCYOX1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2032714
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MBS2032714 is an antibody targeting PCYOX1. Also known as Prenylcysteine Oxidase 1 (PCYOX1) Polyclonal Antibody. Reactive in Human, Mouse. Host: Rabbit. More details

$215 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Prenylcysteine Oxidase 1 (PCYOX1), Polyclonal Antibody
Full Product Name
Prenylcysteine Oxidase 1 (PCYOX1) Polyclonal Antibody
Product Gene Name
Product Synonym Names
PCL1; Prenylcysteine lyase
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Purity/Purification
Affinity Chromatography
Concentration
500ug/mL (lot specific)
Specificity
The antibody is a rabbit polyclonal antibody raised against PCYOX1. It has been selected for its ability to recognize PCYOX1 in immunohistochemical staining and western blotting.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Matching Pairs
Unconjugated Antibody: PCYOX1 (MBS2032714)
Immunogen: PCYOX1 (MBS2033210)
Matching Pairs
Unconjugated Antibody: PCYOX1 (MBS2032714)
APC-CY7 Conjugated Antibody: PCYOX1 (MBS2079776)
Molecular Weight
48,507 Da
OMIM
AF181490 mRNA
3D Structure
ModBase 3D Structure for Q9UHG3
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Human, Mouse
Organism Species
Human
Fragment
PCYOX1 (Tyr174~Leu505)
Quality Control
Content: The quality control contains recombinant PCYOX1 (Tyr174~Leu505) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2079778
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-PCYOX1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-PCYOX1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-PCYOX1 antibody

Western Blot: Sample: Mouse Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Rabbit Anti-PCYOX1 Polyclonal Antibody – Cat# MBS2032714

Western Blot (WB) of anti-PCYOX1 antibody

Western Blot: Sample: Recombinant protein.
Rabbit Anti-PCYOX1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2032714

Immunohistochemistry (IHC) of anti-PCYOX1 antibody

DAB staining on IHC-P; Samples: Human Liver Tissue)
Rabbit Anti-PCYOX1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS2032714

Knockout Validation of anti-PCYOX1 antibody

Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: PCYOX1 knockout Hela cell lysate; Predicted MW: 49,57kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human PCYOX1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
Rabbit Anti-PCYOX1 Polyclonal Antibody, Knockout Validation – Cat# MBS2032714

Other Notes

Small volumes of anti-PCYOX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PCYOX1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
prenylcysteine oxidase 1
NCBI Official Synonym Full Names
prenylcysteine oxidase 1
NCBI Official Symbol
PCYOX1
NCBI Official Synonym Symbols
PCL1
UniProt Protein Name
Prenylcysteine oxidase 1
UniProt Synonym Protein Names
Prenylcysteine lyase
UniProt Gene Name
PCYOX1
UniProt Synonym Gene Names
KIAA0908; PCL1
NCBI Protein Information
prenylcysteine oxidase 1

NCBI Summary for PCYOX1

Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product (Tschantz et al., 1999 [PubMed 10585463]).[supplied by OMIM, Mar 2008]

UniProt Comments for PCYOX1

PCYOX1: Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Belongs to the prenylcysteine oxidase family.

Protein type: EC 1.8.3.5; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: lysosome; plasma membrane; vacuolar membrane

Molecular Function: chloride-transporting ATPase activity; prenylcysteine oxidase activity

Biological Process: prenylated protein catabolic process; prenylcysteine catabolic process; prenylcysteine metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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