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eIF4E-BP1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203380

eIF4E-BP1, also known as eukaryotic translation initiation factor 4E-binding protein 1, is a member of a family of translation repressor proteins. This protein regulates eukaryotic translation initiation factor 4E (eIF4E) activity by preventing its assembly… More details

$210 / 0.02 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
eIF4E-BP1, Recombinant Protein
Full Product Name
eIF4E-BP1, 1-118aa, Human, His tag, E Coli
Product Synonym Names
Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; 4EBP1; BP-1; MGC4316; PHAS-I; Eukaryotic translation initiation factor 4E-binding protein 1; EIF4EBP1; eukaryotic translation initiation factor 4E binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
Sequence
MGSSHHHHHH SSGLVPRGSH MSGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLM ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI
Host
E Coli
Form/Format
Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by BCA assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
14.7 kDa (138aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)
OMIM
L36055 mRNA
3D Structure
ModBase 3D Structure for Q13541
Antigen Species
Human
Tag Information
His-tag
Applications Tested/Suitable for eIF4E-BP1 recombinant protein
SDS-PAGE

Product References and Citations for eIF4E-BP1 recombinant protein

Pause A., et al. (1994) Nature. 371:762-767.; Olsen JV., et al. (2006) Cell. 127 (3):635-48.;

Other Notes

Small volumes of eIF4E-BP1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eIF4E-BP1 purchase

MBS203380 is a recombinant protein targeting eIF4E-BP1. Also known as eIF4E-BP1, 1-118aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF4E-BP1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UniProt Gene Name
EIF4EBP1
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1

NCBI Summary for eIF4E-BP1

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
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UniProt Comments for eIF4E-BP1

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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