AKR7A2 Active Protein

Name: AKR7A2 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203414
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203414

Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver… More details

$245 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

AKR7A2, Active Protein

Full Product Name

AKR7A2, 1-359aa, Human, His tag, E. coli (Bioactivity Validated)

Product Gene Name

AKR7A2 active protein

Product Synonym Names

aldo-keto reductase family 7 member A2; Aflatoxin B1 aldehyde reductase member 2; AFAR; AFAR1; AFB1-AR1; AKR7; aldo-keto reductase family 7; member A2 AFB1 AR1; AFB1AR1; aflatoxin aldehyde reductase; aflatoxin beta1 aldehyde reductase; Aiar; aldo keto reductase family 7; aldo keto reductase family 7; member A2; aldoketoreductase 7.

Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

44.0kDa (398aa), confirmed by MALDI-TOF.

OMIM

AL035413 Genomic DNA

3D Structure

ModBase 3D Structure for O43488

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity is > 1000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the reduction 1.0 pmole of 1,2-Naphthoquinone presence of NADPH per minute at pH 7.0 at 25C.

Product Categories/Family for AKR7A2 active protein

Redox Proteins

Applications Tested/Suitable for AKR7A2 active protein

SDS-PAGE

Product References and Citations for AKR7A2 active protein

Bodreddigari S., et al. (2008) Chem Res Toxicol. 21 (5):1134-42.; Malaspina P., et al. (2009) Hum Genomics. 3 (2):106-20.

Other Notes

Small volumes of AKR7A2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AKR7A2 purchase

MBS203414 is an active protein targeting AKR7A2. Also known as AKR7A2, 1-359aa, Human, His tag, E. coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for AKR7A2. It may not necessarily be applicable to this product.

NCBI GI #

41327764

NCBI GeneID

8574

NCBI Accession #

NP_003680

UniProt Primary Accession #

O43488

UniProt Secondary Accession #

O75749; Q5TG63

UniProt Related Accession #

O43488

GenBank Nucleotide #

NM_003689.3

NCBI Official Full Name

aflatoxin B1 aldehyde reductase member 2 isoform 1

NCBI Official Synonym Full Names

aldo-keto reductase family 7 member A2

NCBI Official Symbol

AKR7A2

NCBI Official Synonym Symbols

AFAR; AKR7; AFAR1; AFB1-AR1

UniProt Protein Name

Aflatoxin B1 aldehyde reductase member 2

UniProt Synonym Protein Names

AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase

Protein Family

Aflatoxin B1 aldehyde reductase

UniProt Gene Name

AKR7A2

UniProt Synonym Gene Names

AFAR; AFAR1; AKR7; AFB1-AR 1; SSA reductase

NCBI Protein Information

aflatoxin B1 aldehyde reductase member 2

NCBI Summary for AKR7A2

The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

UniProt Comments for AKR7A2

Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2-nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen.

Research Articles on AKR7A2

1. AKR7A2 physically interacts with CYGB.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AKR7A2 active protein

Reference Product	PubMed Publications
AKR1A1 active protein	>5 publications with AKR7A2 and AKR1A1
AKR7A3 active protein	>5 publications with AKR7A2 and AKR7A3
AKR1B1 active protein	>4 publications with AKR7A2 and AKR1B1
JUN active protein	>3 publications with AKR7A2 and JUN
NFE2L2 active protein	>2 publications with AKR7A2 and NFE2L2

Pathways associated with AKR7A2 active protein

Products by Pathway	Pathway Diagram
Aflatoxin B1 Metabolism Pathway antibodies	Aflatoxin B1 Metabolism Pathway Diagram
Aflatoxin Activation And Detoxification Pathway antibodies	Aflatoxin Activation And Detoxification Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies	Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
Metabolism Of Xenobiotics By Cytochrome P450 Pathway antibodies	Metabolism Of Xenobiotics By Cytochrome P450 Pathway Diagram
Metapathway Biotransformation antibodies	Metapathway Biotransformation Diagram

Diseases associated with AKR7A2 active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>4 publications with AKR7A2 and Nervous System Diseases
Renal Insufficiency Antibodies	>1 publications with AKR7A2 and Renal Insufficiency

Organs/Tissues associated with AKR7A2 active protein

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>9 publications with AKR7A2 and Liver
Muscle Antibodies	>8 publications with AKR7A2 and Muscle
Brain Antibodies	>6 publications with AKR7A2 and Brain
Kidney Antibodies	>5 publications with AKR7A2 and Kidney
Heart Antibodies	>5 publications with AKR7A2 and Heart
Blood Antibodies	>3 publications with AKR7A2 and Blood
Testis Antibodies	>3 publications with AKR7A2 and Testis
Lung Antibodies	>3 publications with AKR7A2 and Lung
Pancreas Antibodies	>2 publications with AKR7A2 and Pancreas
Spleen Antibodies	>2 publications with AKR7A2 and Spleen