TYMS Recombinant Protein – Cat# MBS203615

3 ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Thymidylate Synthase (TYMS) Recombinant Protein

Name: TYMS Recombinant Protein; Thymidylate Synthase; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203615
Price: 220 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203615

Thymidylate synthase is an intracellular enzyme critical for de novo synthesis of DNA. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. In cancer, expression of this protein is often elevated and… More details

$220 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Thymidylate synthase (TYMS), Recombinant Protein

Full Product Name

Thymidylate synthase, 1-313aa, Human, His tag, E Coli

Product Gene Name

TYMS recombinant protein

Product Synonym Names

HST422; TMS; TS; d TMP synthase; EC 2.1.1.45; MGC88736; Thymidylate synthase; Thymidylate synthetase; Tsase; TYMS; TYMS protein; Tyms thymidylate synthetase

Sequence

MGSSHHHHHH SSGLVPRGSH MPVAGSELPR RPLPPAAQER DAEPRPPHGE LQYLGQIQHI LRCGVRKDDR TGTGTLSVFG MQARYSLRDE FPLLTTKRVF WKGVLEELLW FIKGSTNAKE LSSKGVKIWD ANGSRDFLDS LGFSTREEGD LGPVYGFQWR HFGAEYRDME SDYSGQGVDQ LQRVIDTIKT NPDDRRIIMC AWNPRDLPLM ALPPCHALCQ FYVVNSELSC QLYQRSGDMG LGVPFNIASY ALLTYMIAHI TGLKPGDFIH TLGDAHIYLN HIEPLKIQLQ REPRPFPKLR ILRKVEKIDD FKAEDFQIEG YNPHPTIKME MAV

Host

E Coli

Form/Format

Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1 M NaCl.

Purity/Purification

> 95% by SDS-PAGE

Concentration

1 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles

Molecular Weight

37.8 kDa (333aa), confirmed by MALDI-TOF.

OMIM

X02308 mRNA

3D Structure

ModBase 3D Structure for P04818

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for TYMS recombinant protein

Enzymes & Proteases

Applications Tested/Suitable for TYMS recombinant protein

SDS-PAGE

Product References and Citations for TYMS recombinant protein

Chiu TH., et al. (2009) Anticancer Res. 29 (11):4503-11. ; Pena MM., et al. (2009) J Biol Chem. 284 (46):31597-607.;

Other Notes

Small volumes of TYMS recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TYMS purchase

MBS203615 is a recombinant protein targeting TYMS. Also known as Thymidylate synthase, 1-313aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TYMS. It may not necessarily be applicable to this product.

NCBI GI #

4507751

NCBI GeneID

7298

NCBI Accession #

NP_001062

UniProt Primary Accession #

P04818

UniProt Secondary Accession #

Q8WYK3; Q8WYK4

UniProt Related Accession #

P04818

GenBank Nucleotide #

NM_001071.2

NCBI Official Full Name

thymidylate synthase

NCBI Official Synonym Full Names

thymidylate synthetase

NCBI Official Symbol

TYMS

NCBI Official Synonym Symbols

TS; TMS; HST422

UniProt Protein Name

Thymidylate synthase

Protein Family

Thymidylate synthase

UniProt Gene Name

TYMS

UniProt Synonym Gene Names

TS; TS; TSase

NCBI Protein Information

thymidylate synthase

NCBI Summary for TYMS

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

UniProt Comments for TYMS

Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.

Research Articles on TYMS

1. High expression level of TYMS is associated with neuroendocrine lung tumors.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TYMS recombinant protein

Reference Product	PubMed Publications
DHFR recombinant protein	>48 publications with TYMS and DHFR
SHMT1 recombinant protein	>32 publications with TYMS and SHMT1
MTHFD1 recombinant protein	>28 publications with TYMS and MTHFD1
FPGS recombinant protein	>15 publications with TYMS and FPGS
TK1 recombinant protein	>7 publications with TYMS and TK1
DTYMK recombinant protein	>3 publications with TYMS and DTYMK
RRM2 recombinant protein	>2 publications with TYMS and RRM2
DCTD recombinant protein	>2 publications with TYMS and DCTD
DHFRL1 recombinant protein	>1 publications with TYMS and DHFRL1
DUT recombinant protein	>1 publications with TYMS and DUT

Pathways associated with TYMS recombinant protein

Products by Pathway	Pathway Diagram
Antifolate Resistance Pathway antibodies	Antifolate Resistance Pathway Diagram
Antifolate Resistance Pathway antibodies	Antifolate Resistance Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Circadian Rythm Related Genes Pathway antibodies	Circadian Rythm Related Genes Pathway Diagram
E2F Mediated Regulation Of DNA Replication Pathway antibodies	E2F Mediated Regulation Of DNA Replication Pathway Diagram
E2F Transcription Factor Network Pathway antibodies	E2F Transcription Factor Network Pathway Diagram
Fluoropyrimidine Activity Pathway antibodies	Fluoropyrimidine Activity Pathway Diagram
G1/S Transition Pathway antibodies	G1/S Transition Pathway Diagram
G1/S-Specific Transcription Pathway antibodies	G1/S-Specific Transcription Pathway Diagram

Diseases associated with TYMS recombinant protein

Disease Name	Pubmed Publications
Digestive System Neoplasms Antibodies	>121 publications with TYMS and Digestive System Neoplasms
Colorectal Neoplasms Antibodies	>87 publications with TYMS and Colorectal Neoplasms
Adenocarcinoma Antibodies	>44 publications with TYMS and Adenocarcinoma
Breast Neoplasms Antibodies	>42 publications with TYMS and Breast Neoplasms
Lung Neoplasms Antibodies	>39 publications with TYMS and Lung Neoplasms
Carcinoma, Squamous Cell Antibodies	>26 publications with TYMS and Carcinoma, Squamous Cell
Neoplasms, Experimental Antibodies	>23 publications with TYMS and Neoplasms, Experimental
Head and Neck Neoplasms Antibodies	>22 publications with TYMS and Head and Neck Neoplasms
Diarrhea Antibodies	>19 publications with TYMS and Diarrhea
Stomach Neoplasms Antibodies	>18 publications with TYMS and Stomach Neoplasms

Organs/Tissues associated with TYMS recombinant protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>92 publications with TYMS and Blood
Lung Antibodies	>50 publications with TYMS and Lung
Stomach Antibodies	>18 publications with TYMS and Stomach
Liver Antibodies	>12 publications with TYMS and Liver
Vascular Antibodies	>10 publications with TYMS and Vascular
Heart Antibodies	>9 publications with TYMS and Heart
Pancreas Antibodies	>8 publications with TYMS and Pancreas
Lymph Antibodies	>7 publications with TYMS and Lymph
Lymph Node Antibodies	>7 publications with TYMS and Lymph Node
Bladder Antibodies	>6 publications with TYMS and Bladder