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PKM2 Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203644

PKM2 is an isoenzyme of the glycolytic enzyme pyruvate kinase. This protein catalyzes the production of pyruvate and ATP from phosphoenolpyruvate. PKM2 interacting with Opa proteins, a bacterial outer membrane protein involved in gonococcal adherence to and… More details

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Product Overview

Online Datasheet in PDF
Product Name
PKM2, Active Protein
Full Product Name
PKM2, 1-531aa, Human, His tag, E Coli (Bioactivity Validated)
Product Gene Name
PKM2 active protein
Product Synonym Names
pyruvate kinase muscle isoform M2; CTHBP; THBP1; p58; M2-PK; Tumor M2-PK; PK2; PK3; PKM; pyruvate kinase; muscle isoform M2 OIP3; PKM; TCB; OPA interacting protein 3; PKM2; Cytosolic thyroid hormone binding protein; Pyruvate kinase 2/3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle; Thyroid hormone binding protein cytosolic; Tumour Type M2 Pyruvate Kinase; Tumor Type M2 Pyruvate Kinase
Sequence
MGSSHHHHHH SSGLVPRGSH MSKPHSEAGT AFIQTQQLHA AMADTFLEHM CRLDIDSPPI TARNTGIICT IGPASRSVET LKEMIKSGMN VARLNFSHGT HEYHAETIKN VRTATESFAS DPILYRPVAV ALDTKGPEIR TGLIKGSGTA EVELKKGATL KITLDNAYME KCDENILWLD YKNICKVVEV GSKIYVDDGL ISLQVKQKGA DFLVTEVENG GSLGSKKGVN LPGAAVDLPA VSEKDIQDLK FGVEQDVDMV FASFIRKASD VHEVRKVLGE KGKNIKIISK IENHEGVRRF DEILEASDGI MVARGDLGIE IPAEKVFLAQ KMMIGRCNRA GKPVICATQM LESMIKKPRP TRAEGSDVAN AVLDGADCIM LSGETAKGDY PLEAVRMQHL IAREAEAAIY HLQLFEELRR LAPITSDPTE ATAVGAVEAS FKCCSGAIIV LTKSGRSAHQ VARYRPRAPI IAVTRNPQTA RQAHLYRGIF PVLCKDPVQE AWAEDVDLRV NFAMNVGKAR GFFKKGDVVI VLTGWRPGSG FTNTMRVVPV P
Host
E Coli
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
60.1 kDa (551aa)
OMIM
M23725 mRNA
3D Structure
ModBase 3D Structure for P14618
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Antigen Species
Human
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Specific activity: > 25,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho (enol)pyruvate to pyruvate per minute at pH 7.5 at 37C.

Product Categories/Family for PKM2 active protein

Cancer
Applications Tested/Suitable for PKM2 active protein
SDS-PAGE

SDS-Page of PKM2 active protein

PKM2 Active Protein – Cat# MBS203644

Product References and Citations for PKM2 active protein

Zwerschke W., et al. (2009). Exp Cell Res. 315 (16):2765-74; Mazurek S., et al. (2007). Ernst Schering Found Symp Proc. (4):99-124.;

Other Notes

Small volumes of PKM2 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PKM2 purchase

MBS203644 is an active protein targeting PKM2. Also known as PKM2, 1-531aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PKM2. It may not necessarily be applicable to this product.

NCBI GI #
33286418
NCBI GeneID
5315
NCBI Accession #
NP_002645
UniProt Primary Accession #
P14618
UniProt Secondary Accession #
P14786; Q53GK4; Q96E76; Q9BWB5; Q9UCV6; Q9UPF2; A6NFK3; B2R5N8; B3KRY0; B4DFX8; B4DUU6
UniProt Related Accession #
P14618
GenBank Nucleotide #
NM_002654.5
NCBI Official Full Name
pyruvate kinase PKM isoform a
NCBI Official Synonym Full Names
pyruvate kinase M1/2
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
NCBI Protein Information
pyruvate kinase PKM

NCBI Summary for PKM2

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
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UniProt Comments for PKM2

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
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Research Articles on PKM2

1. In this study, we indicate that P53 (N340Q/L344R) promotes hepatocarcinogenesis through upregulation of PKM2

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PKM2 active protein

 Reference Product  PubMed Publications
 ENO1 active protein  >6 publications with PKM2 and ENO1
 ALDOA active protein  >3 publications with PKM2 and ALDOA
 LDHB active protein  >3 publications with PKM2 and LDHB
 GPI active protein  >3 publications with PKM2 and GPI
 TPI1 active protein  >1 publications with PKM2 and TPI1
 PGK1 active protein  >1 publications with PKM2 and PGK1
 ENO2 active protein  >1 publications with PKM2 and ENO2

Pathways associated with PKM2 active protein

 Products by Pathway  Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies  Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
 Amino Acid Metabolism Pathway antibodies  Amino Acid Metabolism Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Central Carbon Metabolism In Cancer Pathway antibodies  Central Carbon Metabolism In Cancer Pathway Diagram
 Glucagon Signaling Pathway antibodies  Glucagon Signaling Pathway Diagram

Diseases associated with PKM2 active protein

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >17 publications with PKM2 and Carcinoma
 Lung Diseases Antibodies  >11 publications with PKM2 and Lung Diseases
 Cardiovascular Diseases Antibodies  >11 publications with PKM2 and Cardiovascular Diseases
 Inflammation Antibodies  >11 publications with PKM2 and Inflammation
 Fibrosis Antibodies  >9 publications with PKM2 and Fibrosis
 Cell Transformation, Neoplastic Antibodies  >9 publications with PKM2 and Cell Transformation, Neoplastic
 Liver Diseases Antibodies  >8 publications with PKM2 and Liver Diseases
 Necrosis Antibodies  >6 publications with PKM2 and Necrosis
 Liver Neoplasms Antibodies  >5 publications with PKM2 and Liver Neoplasms
 Disease Models, Animal Antibodies  >5 publications with PKM2 and Disease Models, Animal

Organs/Tissues associated with PKM2 active protein

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >75 publications with PKM2 and Brain
 Blood Antibodies  >49 publications with PKM2 and Blood
 Muscle Antibodies  >47 publications with PKM2 and Muscle
 Thyroid Antibodies  >23 publications with PKM2 and Thyroid
 Liver Antibodies  >21 publications with PKM2 and Liver
 Kidney Antibodies  >20 publications with PKM2 and Kidney
 Heart Antibodies  >15 publications with PKM2 and Heart
 Vascular Antibodies  >13 publications with PKM2 and Vascular
 Nerve Antibodies  >12 publications with PKM2 and Nerve
 Lung Antibodies  >11 publications with PKM2 and Lung
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