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Fibronectin 1 (FN1) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600545

Description: FN1 Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Mostly… More details

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Product Overview

Online Datasheet in PDF
Product Name
Fibronectin 1 (FN1), Polyclonal Antibody
Full Product Name
Fibronectin 1 Antibody
Product Gene Name
anti-FN1 antibody
Product Synonym Names
CIG; Cold insoluble globulin; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; Fibronectin 1; FINC; FINC_HUMAN; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Ugl-Y3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
Fibronectin 1 antibody detects endogenous levels of Fibronectin 1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
Fibronectin peptide (MBS9615098) is used for blocking the activity of Fibronectin antibody (MBS9600545)
Molecular Weight
Observed: 260 kDa
Predicted: 263 kDa
3D Structure
ModBase 3D Structure for P02751
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human Fibronectin 1
Subcellular Location
Secreted, Extracellular Space, Extracellular Matrix.
Tissue Specificity
Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-FN1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-FN1 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-FN1 antibody

MBS9600545 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-FN1 Polyclonal Antibody – Cat# MBS9600545

Western Blot (WB) of anti-FN1 antibody

Western blot analysis on HeLa cell lysate using Fibronectin 1 Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-FN1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9600545

Immunohistochemistry (IHC) of anti-FN1 antibody

MBS9600545 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Rabbit Anti-FN1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9600545

Other Notes

Small volumes of anti-FN1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FN1 purchase

MBS9600545 is an antibody targeting FN1. Also known as Fibronectin 1 Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FN1. It may not necessarily be applicable to this product.

NCBI GI #
807066342
NCBI GeneID
2335
NCBI Accession #
NP_001293058.1
UniProt Primary Accession #
P02751
UniProt Secondary Accession #
O95609; O95610; Q14312; Q14325; Q14326; Q17RV7; Q564H7; Q585T2; B7ZLF0; E9PE77; E9PG29
UniProt Related Accession #
P02751
GenBank Nucleotide #
NM_001306129.1
NCBI Official Full Name
fibronectin isoform 8
NCBI Official Synonym Full Names
fibronectin 1
NCBI Official Symbol
FN1
NCBI Official Synonym Symbols
FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
UniProt Protein Name
Fibronectin
UniProt Synonym Protein Names
Cold-insoluble globulin; CIG
Protein Family
Fibronectin
UniProt Gene Name
FN1
UniProt Synonym Gene Names
FN; FN; CIG
NCBI Protein Information
fibronectin

NCBI Summary for FN1

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
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UniProt Comments for FN1

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
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Research Articles on FN1

1. TGFB1-mediated PI3K/Akt and p38 MAP kinase dependent alternative splicing of fibronectin extra domain A in human podocyte culture has been reported.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FN1 antibody

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 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Bacterial Invasion Of Epithelial Cells Pathway antibodies  Bacterial Invasion Of Epithelial Cells Pathway Diagram
 Cell Surface Interactions At The Vascular Wall Pathway antibodies  Cell Surface Interactions At The Vascular Wall Pathway Diagram

Diseases associated with anti-FN1 antibody

 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >83 publications with FN1 and Carcinoma
 Fibrosis Antibodies  >38 publications with FN1 and Fibrosis
 Inflammation Antibodies  >32 publications with FN1 and Inflammation
 Lung Diseases Antibodies  >28 publications with FN1 and Lung Diseases
 Cardiovascular Diseases Antibodies  >23 publications with FN1 and Cardiovascular Diseases
 Lung Neoplasms Antibodies  >23 publications with FN1 and Lung Neoplasms
 Breast Neoplasms Antibodies  >22 publications with FN1 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >21 publications with FN1 and Neoplasms, Experimental
 Neoplasm Invasiveness Antibodies  >19 publications with FN1 and Neoplasm Invasiveness
 Kidney Diseases Antibodies  >17 publications with FN1 and Kidney Diseases

Organs/Tissues associated with anti-FN1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >66 publications with FN1 and Blood
 Muscle Antibodies  >38 publications with FN1 and Muscle
 Lung Antibodies  >37 publications with FN1 and Lung
 Vascular Antibodies  >35 publications with FN1 and Vascular
 Liver Antibodies  >30 publications with FN1 and Liver
 Bone Antibodies  >30 publications with FN1 and Bone
 Kidney Antibodies  >30 publications with FN1 and Kidney
 Embryonic Tissue Antibodies  >23 publications with FN1 and Embryonic Tissue
 Brain Antibodies  >21 publications with FN1 and Brain
 Connective Tissue Antibodies  >21 publications with FN1 and Connective Tissue
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