PDE6D Recombinant Protein

Name: PDE6D Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203656
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203656

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (alphabeta) that is regulated by two inhibitory gamma subunits. As PDE6D does not modify the catalytic… More details

$245 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

PDE6D, Recombinant Protein

Full Product Name

PDE6D, 1-150aa, Human, His tag, E Coli

Product Gene Name

PDE6D recombinant protein

Product Synonym Names

Retinal rod rhodopsin-sensitive cGMP 3'5'-cyclic phosphodiesterase subunit delta; PDED; Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta; phosphodiesterase 6D; cGMP-specific; rod; delta

Sequence

MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH

Host

E Coli

Form/Format

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.5 mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

18.4 kDa (158aa), confirmed by MALDI-TOF

OMIM

AF045999 Genomic DNA

3D Structure

ModBase 3D Structure for O43924

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for PDE6D recombinant protein

Signal Transduction

Applications Tested/Suitable for PDE6D recombinant protein

SDS-PAGE

Product References and Citations for PDE6D recombinant protein

Zhang H., et al, (2007) Proc Natl Acad Sci u S A. 104 (21):8857-62.; Dekomien., G. et al. (2003) Genet Sel Evol. 35 (4):445-56.;

Other Notes

Small volumes of PDE6D recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PDE6D purchase

MBS203656 is a recombinant protein targeting PDE6D. Also known as PDE6D, 1-150aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for PDE6D. It may not necessarily be applicable to this product.

NCBI GI #

4505671

NCBI GeneID

5147

NCBI Accession #

NP_002592

UniProt Primary Accession #

O43924

UniProt Secondary Accession #

O43250

UniProt Related Accession #

O43924

GenBank Nucleotide #

NM_002601.3

NCBI Official Full Name

retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 1

NCBI Official Synonym Full Names

phosphodiesterase 6D

NCBI Official Symbol

PDE6D

NCBI Official Synonym Symbols

PDED; JBTS22

UniProt Protein Name

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

UniProt Synonym Protein Names

Protein p17

Protein Family

Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase

UniProt Gene Name

PDE6D

UniProt Synonym Gene Names

PDED; GMP-PDE delta

NCBI Protein Information

retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta

NCBI Summary for PDE6D

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

UniProt Comments for PDE6D

Promotes the release of prenylated target proteins from cellular membranes (PubMed:9712853). Modulates the activity of prenylated or palmitoylated Ras family members by regulating their subcellular location (PubMed:22002721, PubMed:23698361). Required for normal ciliary targeting of farnesylated target proteins, such as INPP5E (PubMed:24166846). Modulates the subcellular location of target proteins by acting as a GTP specific dissociation inhibitor (GDI) (). Increases the affinity of ARL3 for GTP by several orders of magnitude. Stabilizes ARL3-GTP by decreasing the nucleotide dissociation rate ().

Research Articles on PDE6D

1. Studies indicate that the binding of UNC119 and PDE6D, to the lipid-modified ciliary cargo and the specific release of the cargo in the cilia by the ciliary small G-protein Arl3 in a GTP-dependent manner.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PDE6D recombinant protein

Reference Product	PubMed Publications
ARL3 recombinant protein	>11 publications with PDE6D and ARL3
RPGR recombinant protein	>5 publications with PDE6D and RPGR
INPP5E recombinant protein	>4 publications with PDE6D and INPP5E
ADCY3 recombinant protein	>1 publications with PDE6D and ADCY3
ARL13B recombinant protein	>1 publications with PDE6D and ARL13B

Pathways associated with PDE6D recombinant protein

Products by Pathway	Pathway Diagram
ARL13B-mediated Ciliary Trafficking Of INPP5E Pathway antibodies	ARL13B-mediated Ciliary Trafficking Of INPP5E Pathway Diagram
Cargo Trafficking To The Periciliary Membrane Pathway antibodies	Cargo Trafficking To The Periciliary Membrane Pathway Diagram
Cilium Assembly Pathway antibodies	Cilium Assembly Pathway Diagram
Organelle Biogenesis And Maintenance Pathway antibodies	Organelle Biogenesis And Maintenance Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram

Diseases associated with PDE6D recombinant protein

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>4 publications with PDE6D and Disease Models, Animal
Atrophy Antibodies	>3 publications with PDE6D and Atrophy
Nervous System Diseases Antibodies	>1 publications with PDE6D and Nervous System Diseases
Kidney Diseases Antibodies	>1 publications with PDE6D and Kidney Diseases

Organs/Tissues associated with PDE6D recombinant protein

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>18 publications with PDE6D and Eye
Brain Antibodies	>3 publications with PDE6D and Brain
Lung Antibodies	>2 publications with PDE6D and Lung
Kidney Antibodies	>2 publications with PDE6D and Kidney
Spleen Antibodies	>1 publications with PDE6D and Spleen
Liver Antibodies	>1 publications with PDE6D and Liver
Blood Antibodies	>1 publications with PDE6D and Blood