AK1 Active Protein

Name: AK1 Active Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS203814
Price: 230 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS203814

AK1 is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of the terminal phosphate group between ATP and AMP. This protein is found in the cytosol of skeletal muscle, brain and… More details

$230 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

AK1, Active Protein

Full Product Name

AK1, 1-194aa, Human, His tag, E Coli (Bioactivity Validated)

Product Gene Name

AK1 active protein

Product Synonym Names

Adenylate kinase isoenzyme1; ATP-AMP transphosphorylase 1; Myokinase

Sequence

MGSSHHHHHH SSGLVPRGSH MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRSEVSSG SARGKKLSEI MEKGQLVPLE TVLDMLRDAM VAKVNTSKGF LIDGYPREVQ QGEEFERRIG QPTLLLYVDA GPETMTQRLL KRGETSGRVD DNEETIKKRL ETYYKATEPV IAFYEKRGIV RKVNAEGSVD SVFSQVCTHL DALK

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% glycerol

Purity/Purification

> 95% by SDS-PAGE

Concentration

1mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

23.7 kDa (214aa) confirmed by MALDI-TOF

OMIM

J04809 Genomic DNA

3D Structure

ModBase 3D Structure for P00568

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Antigen Species

Human

Tag Information

His-tag

Biological Activity

Specific activity: > 600 units/mg. One unit will convert 2.0 umoles of ADP to ATP + AMP per minute at pH 7.5 at 37C.

Product Categories/Family for AK1 active protein

Metabolism

Applications Tested/Suitable for AK1 active protein

SDS-PAGE

Product References and Citations for AK1 active protein

Terzic A., et al. (2007) J Biol Chem. 282 (43):31366-72.; Morelli A., et al (2007) Curr Eye Res. 32 (3):249-57.;

Other Notes

Small volumes of AK1 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AK1 purchase

MBS203814 is an active protein targeting AK1. Also known as AK1, 1-194aa, Human, His tag, E Coli (Bioactivity Validated). Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for AK1. It may not necessarily be applicable to this product.

NCBI GI #

4502011

NCBI GeneID

203

NCBI Accession #

NP_000467

UniProt Primary Accession #

P00568

UniProt Secondary Accession #

Q9BVK9; Q9UQC7

UniProt Related Accession #

P00568

GenBank Nucleotide #

NM_000476.2

NCBI Official Full Name

adenylate kinase isoenzyme 1 isoform 1

NCBI Official Synonym Full Names

adenylate kinase 1

NCBI Official Symbol

AK1

NCBI Official Synonym Symbols

HTL-S-58j

UniProt Protein Name

Adenylate kinase isoenzyme 1

UniProt Synonym Protein Names

ATP-AMP transphosphorylase 1

Protein Family

Adenylate kinase

UniProt Gene Name

AK1

UniProt Synonym Gene Names

AK 1

NCBI Protein Information

adenylate kinase isoenzyme 1

NCBI Summary for AK1

This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

UniProt Comments for AK1

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.

Research Articles on AK1

1. Studies indicate that the preferred substrate and phosphate donor of all adenylate kinases are AMP and ATP respectively.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with AK1 active protein

Reference Product	PubMed Publications
NME4 active protein	>1 publications with AK1 and NME4
NME7 active protein	>1 publications with AK1 and NME7
NME6 active protein	>1 publications with AK1 and NME6
ADSL active protein	>1 publications with AK1 and ADSL
ATP5A1 active protein	>1 publications with AK1 and ATP5A1

Pathways associated with AK1 active protein

Products by Pathway	Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies	Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Nucleotides Pathway antibodies	Metabolism Of Nucleotides Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Purine Metabolism Pathway antibodies	Purine Metabolism Pathway Diagram
Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway antibodies	Synthesis And Interconversion Of Nucleotide Di- And Triphosphates Pathway Diagram
Thiamine Metabolism Pathway antibodies	Thiamine Metabolism Pathway Diagram
Thiamine Metabolism Pathway antibodies	Thiamine Metabolism Pathway Diagram

Diseases associated with AK1 active protein

Disease Name	Pubmed Publications
Heart Diseases Antibodies	>8 publications with AK1 and Heart Diseases
Disease Models, Animal Antibodies	>7 publications with AK1 and Disease Models, Animal
Movement Disorders Antibodies	>6 publications with AK1 and Movement Disorders
Inflammation Antibodies	>4 publications with AK1 and Inflammation
Myocardial Ischemia Antibodies	>4 publications with AK1 and Myocardial Ischemia
Liver Diseases Antibodies	>3 publications with AK1 and Liver Diseases
Nerve Degeneration Antibodies	>2 publications with AK1 and Nerve Degeneration
Growth Disorders Antibodies	>2 publications with AK1 and Growth Disorders
Breast Neoplasms Antibodies	>2 publications with AK1 and Breast Neoplasms
Seizures Antibodies	>2 publications with AK1 and Seizures

Organs/Tissues associated with AK1 active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>98 publications with AK1 and Blood
Muscle Antibodies	>48 publications with AK1 and Muscle
Brain Antibodies	>25 publications with AK1 and Brain
Heart Antibodies	>22 publications with AK1 and Heart
Liver Antibodies	>14 publications with AK1 and Liver
Nerve Antibodies	>10 publications with AK1 and Nerve
Vascular Antibodies	>6 publications with AK1 and Vascular
Kidney Antibodies	>6 publications with AK1 and Kidney
Eye Antibodies	>4 publications with AK1 and Eye
Pancreas Antibodies	>4 publications with AK1 and Pancreas