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Antigen 85a (fbpA) Baculovirus Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204142

Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C). The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord… More details

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Product Overview

Online Datasheet in PDF
Product Name
Antigen 85A (fbpA), Recombinant Protein
Full Product Name
Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus
Product Gene Name
Product Synonym Names
Antigen 85 complex A; Mycolytransferase 85A; Fibronectin-binding protein A; TB Ab85A; TBab85A
Sequence
ADPAFSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGAH HHHHH
Host
Baculovirus
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing, 10% glycerol
Purity/Purification
> 95% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Absorbance at 280nm) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
32.8kDa (305aa)
Antigen Species
Mycobacterium tuberculosis
Tag Information
His-tag
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Applications Tested/Suitable for fbpA recombinant protein
SDS-PAGE

Product References and Citations for fbpA recombinant protein

Elamin AA., et al. (2009) J Microbiol Methods. 79 (3):358-63.; Jeon BY., et al. (2011) Microbes Infect. 13 (3):284-90.

Other Notes

Small volumes of fbpA recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for fbpA purchase

MBS204142 is a recombinant protein targeting fbpA. Also known as Antigen 85A, 43-338aa, Mycobacterium tuberculosis, His tag, Baculovirus. Host: Baculovirus.

Additional Details

NCBI/Uniprot data below describe general gene information for fbpA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
diacylglycerol acyltransferase/mycolyltransferase Ag85A
NCBI Official Symbol
fbpA
NCBI Official Synonym Symbols
85A; mpt44
UniProt Protein Name
Diacylglycerol acyltransferase/mycolyltransferase Ag85A
UniProt Synonym Protein Names
Acyl-CoA:diacylglycerol acyltransferase; Antigen 85 complex A; 85A; Ag85A; Fibronectin-binding protein A; Fbps A
UniProt Gene Name
fbpA
UniProt Synonym Gene Names
mpt44; DGAT; 85A; Ag85A; Fbps A
NCBI Protein Information
diacylglycerol acyltransferase/mycolyltransferase Ag85A

UniProt Comments for fbpA

The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan, and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM. FbpA mediates triacylglycerol (TAG) formation with long-chain acyl-CoA as the acyl donor and 1,2-dipalmitoyl-sn-glycerol (1,2-dipalmitin) as the acyl acceptor. It has a preference for C26:0-CoA over C18:1-CoA.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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