SNF8 Recombinant Protein

Name: SNF8 Recombinant Protein; Research Grade; MyBioSource
Brand: MyBioSource
SKU: MBS204228
Price: 245 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204228

SNF 8, also known as EAP30 (ELL-associated protein of 30 kDa), is localizes to both the nucleus and the cytoplasm and is a member of the SNF8 family of vacuolar sorting proteins. This protein is subunit of the endosomal sorting complex required for transport… More details

$245 / 0.02 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

SNF8, Recombinant Protein

Full Product Name

SNF8, 1-258aa, Human, His tag, E Coli

Product Gene Name

SNF8 recombinant protein

Product Synonym Names

Vacuolar-sorting protein SNF8; Dot3; EAP30; VPS22

Sequence

MGSSHHHHHH SSGLVPRGSH MGSHMHRRGV GAGAIAKKKL AEAKYKERGT VLAEDQLAQM SKQLDMFKTN LEEFASKHKQ EIRKNPEFRV QFQDMCATIG VDPLASGKGF WSEMLGVGDF YYELGVQIIE VCLALKHRNG GLITLEELHQ QVLKGRGKFA QDVSQDDLIR AIKKLKALGT GFGIIPVGGT YLIQSVPAEL NMDHTVVLQL AEKNGYVTVS EIKASLKWET ERARQVLEHL LKEGLAWLDL QAPGEAHYWL PALFTDLYSQ EITAEEAREA LP

Host

E Coli

Form/Format

Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 50% glycerol, 2mM DTT, 200mM NaCl

Purity/Purification

> 90% by SDS-PAGE

Concentration

0.25mg/ml (determined by Bradford assay) (lot specific)

Preparation and Storage

Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

Molecular Weight

31.4kDa (282aa) confirmed by MALDI-TOF

OMIM

AF156102 mRNA

3D Structure

ModBase 3D Structure for Q96H20

Antigen Species

Human

Tag Information

His-tag

Product Categories/Family for SNF8 recombinant protein

Epigenomics

Applications Tested/Suitable for SNF8 recombinant protein

SDS-PAGE

Product References and Citations for SNF8 recombinant protein

Maleroed L., et al. (2007) Traffic 8:1617-1629; Raiborg C., et al. (2008) Exp. Cell Res. 314:801-813

Other Notes

Small volumes of SNF8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for SNF8 purchase

MBS204228 is a recombinant protein targeting SNF8. Also known as SNF8, 1-258aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for SNF8. It may not necessarily be applicable to this product.

NCBI GI #

21361380

NCBI GeneID

11267

NCBI Accession #

NP_009172

UniProt Primary Accession #

Q96H20

UniProt Secondary Accession #

Q8IXY3; Q9UN50

UniProt Related Accession #

Q96H20

GenBank Nucleotide #

NM_007241.3

NCBI Official Full Name

vacuolar-sorting protein SNF8 isoform 1

NCBI Official Synonym Full Names

SNF8, ESCRT-II complex subunit

NCBI Official Symbol

SNF8

NCBI Official Synonym Symbols

Dot3; EAP30; VPS22

UniProt Protein Name

Vacuolar-sorting protein SNF8

UniProt Synonym Protein Names

ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22

Protein Family

Vacuolar-sorting protein

UniProt Gene Name

SNF8

UniProt Synonym Gene Names

EAP30; hVps22

NCBI Protein Information

vacuolar-sorting protein SNF8

NCBI Summary for SNF8

The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

UniProt Comments for SNF8

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. It is also required for the degradation of CXCR4. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Research Articles on SNF8

1. data describe an unappreciated role for EAP30 in IRF3-dependent innate antiviral response in the nucleus.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with SNF8 recombinant protein

Reference Product	PubMed Publications
VPS36 recombinant protein	>9 publications with SNF8 and VPS36
VPS25 recombinant protein	>6 publications with SNF8 and VPS25
TSG101 recombinant protein	>3 publications with SNF8 and TSG101
CHMP3 recombinant protein	>2 publications with SNF8 and CHMP3
VPS28 recombinant protein	>2 publications with SNF8 and VPS28

Pathways associated with SNF8 recombinant protein

Products by Pathway	Pathway Diagram
ESCRT-II Complex Pathway antibodies	ESCRT-II Complex Pathway Diagram
ESCRT-II Complex Pathway antibodies	ESCRT-II Complex Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endocytosis Pathway antibodies	Endocytosis Pathway Diagram
Endosomal Sorting Complex Required For Transport (ESCRT) Pathway antibodies	Endosomal Sorting Complex Required For Transport (ESCRT) Pathway Diagram
Membrane Trafficking Pathway antibodies	Membrane Trafficking Pathway Diagram
Vesicle-mediated Transport Pathway antibodies	Vesicle-mediated Transport Pathway Diagram

Diseases associated with SNF8 recombinant protein

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>2 publications with SNF8 and Kidney Diseases
Immune System Diseases Antibodies	>1 publications with SNF8 and Immune System Diseases
Neoplasm Metastasis Antibodies	>1 publications with SNF8 and Neoplasm Metastasis
Liver Diseases Antibodies	>1 publications with SNF8 and Liver Diseases
Heart Diseases Antibodies	>1 publications with SNF8 and Heart Diseases
Hypertension Antibodies	>1 publications with SNF8 and Hypertension
Hypertrophy Antibodies	>1 publications with SNF8 and Hypertrophy
Hemorrhage Antibodies	>1 publications with SNF8 and Hemorrhage

Organs/Tissues associated with SNF8 recombinant protein

Organ/Tissue Name	Pubmed Publications
Vascular Antibodies	>2 publications with SNF8 and Vascular
Kidney Antibodies	>2 publications with SNF8 and Kidney
Nerve Antibodies	>1 publications with SNF8 and Nerve
Muscle Antibodies	>1 publications with SNF8 and Muscle
Liver Antibodies	>1 publications with SNF8 and Liver
Intestine Antibodies	>1 publications with SNF8 and Intestine