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EYA2 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204489

EYA2, also known as eyes absent homolog 2, is a member of tyrosine phosphatase family that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). This protein promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
EYA2, Recombinant Protein
Full Product Name
EYA2, 244-514aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Eyes absent homolog 2; EAB1; MGC10614
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
0.5 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
33.2 kDa (295aa), confirmed by MALDI-TOF
OMIM
U71207 mRNA
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for EYA2 recombinant protein

Applications Tested/Suitable for EYA2 recombinant protein
SDS-PAGE

Product References and Citations for EYA2 recombinant protein

Krishnan N., et al. (2009) J. Biol. Chem. 284:16066-16070; Fan X., et al. (2000) J. Biol. Chem. 275:32129-32134

Other Notes

Small volumes of EYA2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EYA2 purchase

MBS204489 is a recombinant protein targeting EYA2. Also known as EYA2, 244-514aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for EYA2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Eyes absent homolog 2 (Drosophila)
NCBI Official Synonym Full Names
EYA transcriptional coactivator and phosphatase 2
NCBI Official Symbol
EYA2
NCBI Official Synonym Symbols
EAB1
UniProt Protein Name
Eyes absent homolog 2
Protein Family
UniProt Gene Name
EYA2
UniProt Synonym Gene Names
EAB1
NCBI Protein Information
eyes absent homolog 2

NCBI Summary for EYA2

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may be post-translationally modified and may play a role in eye development. A similar protein in mice can act as a transcriptional activator. Alternative splicing results in multiple transcript variants, but the full-length natures of all of these variants have not yet been determined. [provided by RefSeq, Jul 2009]
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UniProt Comments for EYA2

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed:12500905, PubMed:23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed:19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed:12500905).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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