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TPK1 Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS204774

TPK1, also known as thiamin pyrophosphokinase 1, catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. This protein can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate. Recombinant human TPK1 protein, fused to… More details

$230 / 0.02 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TPK1, Recombinant Protein
Full Product Name
TPK1, 1-243aa, Human, His tag, E Coli
Product Gene Name
Product Synonym Names
Thiamin pyrophosphokinase 1; HTPK1; PP20
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACAD GGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKD LKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLI PVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS
Host
E Coli
Form/Format
Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Purity/Purification
> 90% by SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay) (lot specific)
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
Molecular Weight
29.8 kDa (267aa) confirmed by MALDI-TOF
OMIM
AB028138 mRNA
3D Structure
ModBase 3D Structure for Q9H3S4
Antigen Species
Human
Tag Information
His-tag

Product Categories/Family for TPK1 recombinant protein

Applications Tested/Suitable for TPK1 recombinant protein
SDS-PAGE

Product References and Citations for TPK1 recombinant protein

Nosaka K., et al. (2001) Biochim. Biophys. Acta. 1517:293-297; Zhao R., et al. (2004) Biochim. Biophys. Acta. 1517:320-322

Other Notes

Small volumes of TPK1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TPK1 purchase

MBS204774 is a recombinant protein targeting TPK1. Also known as TPK1, 1-243aa, Human, His tag, E Coli. Host: E Coli.

Additional Details

NCBI/Uniprot data below describe general gene information for TPK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
thiamin pyrophosphokinase 1 isoform a
NCBI Official Synonym Full Names
thiamin pyrophosphokinase 1
NCBI Official Symbol
TPK1
NCBI Official Synonym Symbols
PP20; HTPK1; THMD5
UniProt Protein Name
Thiamin pyrophosphokinase 1
UniProt Synonym Protein Names
Placental protein 20; PP20; Thiamine pyrophosphokinase 1
UniProt Gene Name
TPK1
UniProt Synonym Gene Names
hTPK1; PP20
NCBI Protein Information
thiamin pyrophosphokinase 1

NCBI Summary for TPK1

The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5. [provided by RefSeq, Apr 2017]
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UniProt Comments for TPK1

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate. Can also catalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with TPK1 recombinant protein

 Reference Product  PubMed Publications
 TKT recombinant protein  >1 publications with TPK1 and TKT
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